Identification and characterization of Col plasmids from classical colicin E-producing strains.

Journal of Bacteriology
R WatsonL P Visentin

Abstract

A series of transformants have been derived which carry the Col plasmids from 11 E-colicin-producing strains isolated by Fredericq or Hamon. The ColE plasmids identified included four of type E1, five of type E2, and two of a type designated E4 by Horak (Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 233:58-63, 1975). Strain K317 was shown to carry a ColE7 plasmid and a 2.7-megadalton (Md) ColE2imm plasmid which confers immunity to colicin E2 but not the ability to produce colicin. Other plasmids identified in the colicinogenic isolates were a 3.4-Md ColN plasmid and a 1.3-Md Col plasmid of unknown type. The ColE1 plasmids all continued replicating in cultures treated with chloramphenicol. Strains carrying the ColE4 or ColE3-CA38 plasmid exhibited partial sensitivity to their own colicins and exhibited an unusual clearing-zone morphology when overlaid on stabs of colicin E2- or E7-producing strains. Except for ColE1-K53, ColE1-K47, ColE2-CA42, and ColE7-K317, all of the ColE plasmids were found to be about 4.3 Md in size. ColE2-CA42 and ColE7-K317 are both about 3.9 Md and are the only two plasmids of the E2, E3, E4, and E7 types that did not yield a 0.39-Md deoxyribonucleic acid fragment as an EcoRI...Continue Reading

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Citations

Jul 1, 1984·Bioscience Reports·P C LauL P Visentin
Jan 24, 2006·Journal of Bacteriology·Masaru YaguraTateo Itoh
Jan 1, 1988·Folia Microbiologica·J Smarda, I Sevcíková
Nov 23, 2018·Acta Microbiologica Et Immunologica Hungarica·Dániel GulyásDóra Szabó
Oct 10, 2015·Journal of Molecular Biology·Robert P MorseCelia W Goulding
Jan 1, 1991·Journal of General Microbiology·K F ChakR James
Jun 1, 1990·Molecular & General Genetics : MGG·M N Kolot
Mar 24, 2017·FEMS Microbiology Letters·Fatema CalcuttawalaThandavarayan Ramamurthy
Jun 11, 2002·Journal of Molecular Biology·Emily S CollinsGeoffrey R Moore
Mar 1, 1983·Journal of Bacteriology·R WatsonL P Visentin
Dec 1, 1994·Journal of Bacteriology·S HiragaT Itoh
Jul 6, 2004·Journal of Microbiological Methods·Larry C AnthonyMarcin Filutowicz
Mar 20, 1985·Journal of Molecular Biology·H Masaki, T Ohta

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