Identification and characterization of dominant helper T-cell epitopes in the nucleocapsid protein of severe acute respiratory syndrome coronavirus

Journal of Virology
Jincun ZhaoXiao-Ming Gao

Abstract

By using a series of overlapping synthetic peptides covering 98% of the amino acid sequence of the nucleocapsid protein (NP) of severe acute respiratory syndrome coronavirus (SARS-CoV), four helper T-cell (Th) epitopes (NP11, residues 11 to 25; NP51, residues 51 to 65; NP61, residues 61 to 75; and NP111, residues 111 to 125) in C57BL mice (H-2(b)), four (NP21, residues 21 to 35; NP91, residues 91 to 105; NP331, residues 331 to 345; and NP351, residues 351 to 365) in C3H mice (H-2(k)), and two (NP81, residues 81 to 95; and NP351, residues 351 to 365) in BALB/c mice (H-2(d)) have been identified. All of these peptides were able to stimulate the proliferation of NP-specific T-cell lines or freshly isolated lymph node cells from mice immunized with recombinant NP. Immunization of mice with synthetic peptides containing appropriate Th epitopes elicited strong cellular immunity in vivo, as evidenced by delayed-type hypersensitivity. Priming with the helper peptides (e.g., NP111 and NP351) significantly accelerated the immune response induced by recombinant NP, as determined by the production of NP-specific antibodies. When fused with a conserved neutralizing epitope (SP1143-1157) from the spike protein of SARS-CoV, NP111 and NP351 as...Continue Reading

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Citations

Sep 21, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Milan Surjit, Sunil K Lal
Oct 18, 2008·Biochemical and Biophysical Research Communications·John MarkMary Alice Hefford
Oct 1, 2011·The Journal of General Virology·Shanshan WangZhenghong Yuan
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Oct 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chris Ka-fai LiXiao-Ning Xu
Oct 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fumihiko YasuiMichinori Kohara
Mar 24, 2020·Indian Journal of Pharmacology·Manisha PrajapatBikash Medhi
Jan 20, 2021·The Journal of Experimental Medicine·Zhen ZhuangJincun Zhao
Jan 23, 2021·Advanced Drug Delivery Reviews·Saborni ChakrabortyTaia T Wang
Jul 1, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Jayalakshmi VallamkonduRamesh Kandimalla
Apr 27, 2021·Informatics in Medicine Unlocked·Md Tahsin KhanMohammad A Halim

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