PMID: 9555888Apr 29, 1998Paper

Identification and characterization of epoxide carboxylase activity in cell extracts of Nocardia corallina B276

Journal of Bacteriology
J R Allen, S A Ensign

Abstract

The metabolism of aliphatic epoxides (epoxyalkanes) by the alkene-utilizing actinomycete Nocardia corallina B276 was investigated. Suspensions of N. corallina cells grown with propylene as the carbon source readily degraded propylene and epoxypropane, while suspensions of glucose-grown cells did not. The addition of propylene and epoxypropane to glucose-grown cells resulted in a time-dependent increase in propylene- and epoxypropane-degrading activities that was prevented by the addition of rifampin and chloramphenicol. The expression of alkene- and epoxide-degrading activities was correlated with the high-level expression of several polypeptides not present in extracts of glucose-grown cells. Epoxypropane and epoxybutane degradation by propylene-grown cell suspensions of N. corallina was stimulated by the addition of CO2 and inhibited by the depletion of CO2. Cell extracts catalyzed the carboxylation of epoxypropane to form acetoacetate in a reaction that was dependent on the addition of CO2, NAD+, and a reductant (NADPH or dithiothreitol). In the absence of CO2, epoxypropane was isomerized by cell extracts to form acetone at a rate approximately 10-fold lower than the rate of epoxypropane carboxylation. Methylepoxypropane was...Continue Reading

References

Dec 18, 1991·European Journal of Biochemistry·M H JacobsD B Janssen
Mar 1, 1996·Journal of Bacteriology·J R Allen, S A Ensign
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·M K Sluis, S A Ensign
Nov 1, 1989·Applied and Environmental Microbiology·S HartmansJ A de Bont

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Citations

Jan 13, 2015·Applied and Environmental Microbiology·Samanthi KottegodaMichael Hyman
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·J R AllenS A Ensign
Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·Arathi M KrishnakumarJohn W Peters
Jun 5, 2020·The Biochemical Journal·Florence MusJohn W Peters
Mar 30, 1999·European Journal of Biochemistry·T J SmithH Dalton
Jan 14, 2003·Annual Review of Biochemistry·Scott A Ensign, Jeffrey R Allen
Mar 27, 1999·Journal of Bacteriology·G J PoelarendsD B Janssen

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