PMID: 3329041Jan 1, 1986

Identification and characterization of four new GCD genes in Saccharomyces cerevisiae

Current Genetics
P NiederbergerR Hütter

Abstract

Mutant strains, resistant against the amino acid analogues 5-methyltryptophan, 5-fluorotryptophan and canavanine were isolated, starting with a trp2 leaky auxotrophic strain. Of 10 such strains, only four turned out to be of the "general control derepressed" (gcd) mutant type. Three other isolates were shown to be defective in the general amino acid permease system, while the remaining three strains displayed low spore viability and were not further investigated. Complementation tests amongst the four new gcd-mutant strains, including strain RH558 gcd2-1 isolated earlier, yielded five complementation groups: GCD2, GCD3, GCD4, GCD5, and GCD6. All mutant strains showed a dual phenotype, which was not separable by wild type backcrosses: "constitutive derepression" and "slow growth". Epistasis of all gcd mutations over gcn1-1, gcn2-1 and gcn3-1 was found with respect to both phenotypes, except for gcd5-1, which was lethal in these combinations. On the other hand gcn4-101 was found to be epistatic over all gcd mutations, but only with respect to the "constitutive derepression" phenotype, and not to "slow growth"; again the combination with gcd5-1 was lethal. Mutation gcd2-1 was mapped on chromosome VII, 50 cM from leu1 and 22 cM fro...Continue Reading

References

Dec 15, 1977·Archives of Microbiology·G MiozzariR Hütter
Aug 1, 1978·Journal of Bacteriology·R SumradaT G Cooper
Oct 1, 1978·Analytical Biochemistry·G MiozzariR Hütter
Aug 5, 1975·Journal of Molecular Biology·M WolfnerG R Fink
Jun 20, 1970·Nature
Apr 1, 1982·Genetical Research·I B Barthelmess
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M D PennH Greer
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A G Hinnebusch
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G ThireosH Greer
Jul 1, 1981·Molecular and Cellular Biology·P NiederbergerR Hütter

Citations

Mar 1, 1988·Current Genetics·S Holmberg, J G Petersen
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P P MuellerA G Hinnebusch
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·N P WilliamsT F Donahue
Apr 1, 1993·Molecular Microbiology·V Sophianopoulou, G Diallinas
Jan 1, 1995·Molecular Microbiology·M KünzlerG H Braus
Jul 31, 1990·European Journal of Biochemistry·P P Müller, H Trachsel
Sep 1, 1989·Yeast·R K MortimerJ A Kans
May 16, 2008·FEMS Yeast Research·Olga de SmidtJacobus Albertyn

Related Concepts

Genome Mapping
Crosses, Genetic
Deviation, Epistatic
Genes, Fungal
Genes, Regulator
Cis-Trans Test
Saccharomyces cerevisiae

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