Identification and characterization of G protein-regulated phospholipase C in human myocardium

Journal of Molecular and Cellular Cardiology
P SchnabelM Böhm

Abstract

The generation of the second messengers inositol 1,4,5-trisphosphate (InsP3) and diacylglycerol (DAG) by phosphoinositide-specific phospholipases C (PLCs) is a key mechanism by which many cellular functions such as intracellular calcium handling or growth and differentiation are modulated. In the myocardium, PLC plays a role in the mediation of positive inotropic effects and is possibly involved in the pathogenesis of myocardial hypertrophy. Among the variety of PLC isozymes known, the PLC beta family is regulated by heterotrimeric G proteins. The aim of the present study was to identify and to characterize the PLC beta isoform present in human myocardium. PLC activity in human myocardial membranes was dependent on the presence of Ca2+. Interestingly, PLC was markedly stimulated by GTP gamma S, used as an activator of G proteins. This stimulation was completely abolished by GDP. However, purified alpha-subunits from retinal transducin (alpha 1), used as scavengers of free beta gamma-subunits, did not abolish this effect indicating GTP gamma S stimulation being mediated by G protein alpha-subunits. PLC activity was also stimulated by G protein beta gamma-subunits purified from bovine retina (beta gamma t). This stimulation was c...Continue Reading

Citations

Sep 23, 1997·British Journal of Pharmacology·P SchnabelM Böhm
Oct 25, 2003·Journal of Cardiovascular Pharmacology·Jason D KiltsMadan M Kwatra
Feb 22, 2001·Journal of Molecular and Cellular Cardiology·P S TappiaV Panagia
May 12, 2007·Brain Research·Inmaculada IglesiasMairena Martín
Jul 28, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Ki-Chul HwangYangsoo Jang
Jun 30, 2016·Nature Communications·Stephan LangeElisabeth Ehler
Dec 24, 1997·Hypertension·P SchnabelM Böhm
Jun 22, 1999·Medizinische Klinik·U LaufsJ K Liao
Aug 17, 2000·Biochemical and Biophysical Research Communications·P SchnabelM Böhm

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