PMID: 2502532Aug 1, 1989Paper

Identification and characterization of genes controlled by the sporulation-regulatory gene spo0H in Bacillus subtilis

Journal of Bacteriology
K J JaacksA D Grossman

Abstract

We describe a general strategy for the identification of genes that are controlled by a specific regulatory factor in vivo and the use of this strategy to identify genes in Bacillus subtilis that are controlled by spo0H, a regulatory gene required for the initiation of sporulation. The general strategy makes use of a cloned regulatory gene fused to an inducible promoter to control expression of the regulatory gene and random gene fusions to a reporter gene to monitor expression in the presence and absence of the regulatory gene product. spo0H encodes a sigma factor of RNA polymerase, sigma H, and is required for the extensive reprograming of gene expression during the transition from growth to stationary phase and during the initiation of sporulation. We identified 18 genes that are controlled by sigma H (csh genes) in vivo by monitoring expression of random gene fusions to lacZ, made by insertion mutagenesis with the transposon Tn917lac, in the presence and absence of sigma H. These genes had lower levels of expression in the absence of sigma H than in the presence of sigma H. Patterns of expression of the csh genes during growth and sporulation in wild-type and spo0H mutant cells indicated that other regulatory factors are pr...Continue Reading

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Citations

Jan 1, 1991·Molecular Microbiology·D Dubnau
Feb 19, 2002·Molecular Microbiology·Tran Thu HoaDavid Dubnau
Nov 8, 2005·Microbiology·Serkalem TadessePeter L Graumann
Mar 8, 2012·PLoS Genetics·Norbert S HillPetra Anne Levin
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Jun 21, 2013·Molecular Biology of the Cell·Christian ReimoldPeter L Graumann
Oct 5, 2016·Journal of Bacteriology·Laurel D Wright, Alan D Grossman
Apr 12, 2019·BMC Research Notes·Rogelio Hernández-Tamayo, Peter L Graumann
Nov 8, 2018·Scientific Reports·Thomas C RöschPeter L Graumann
Mar 15, 2020·Applied and Environmental Microbiology·Felix DempwolffDaniel B Kearns
Jul 24, 2004·EMBO Reports·Hervé Joël Defeu Soufo, Peter L Graumann
Mar 12, 2013·Journal of Bacteriology·Luise A K Kleine BorgmannPeter L Graumann
Jan 18, 2011·Journal of Bacteriology·Christine KaimerPeter L Graumann
Feb 3, 2016·Journal of Bacteriology·Christopher M Johnson, Alan D Grossman

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