PMID: 2111805Jun 1, 1990Paper

Identification and characterization of hetA, a gene that acts early in the process of morphological differentiation of heterocysts

Journal of Bacteriology
D Holland, C Peter Wolk

Abstract

Envelope polysaccharide is a major early diagnostic of differentiating heterocysts. The mutation in mutant EF116 of Anabaena sp. strain PCC 7120 reduces the cohesiveness of this polysaccharide. A 3.5-kilobase fragment of DNA cloned from the wild type of this Anabaena sp. was previously shown to complement this mutation. We present the nucleotide sequence of a 2,555-base-pair portion of this fragment containing an open reading frame (ORF) of 601 amino acids. Complementation analysis using deletion derivatives of the 3.5-kilobase fragment showed that the gene mutated in EF116, which we designate hetA, lies within this ORF. Transcription of hetA was induced as a result of deprivation for nitrate and yielded a monocistronic mRNA that was present at greatest abundance 7 h after nitrogen stepdown. At that time, proheterocysts could not be distinguished by light microscopy; transcription of nifHD, structural genes of nitrogenase, peaked much later. Situated 3' to hetA are 4 identical repeats of the sequence 5'-TTCAAAA-3' and 12 repeats (10 identical) of the sequence 5'-CCCCAAT-3'. The 12 repeats, present within and near the 5' end of a second ORF, are almost identical to repeats that have been reported to be present in the region betw...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T KallasR Malkin
Jan 1, 1985·Cold Spring Harbor Symposia on Quantitative Biology·D KaiserA Kuspa
Jan 1, 1988·Methods in Enzymology·J Elhai, C P Wolk
Jan 1, 1986·Annual Review of Genetics·G N GussinF M Ausubel
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R FisherR Haselkorn
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C P WolkJ Elhai
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M D BigginG F Hong
Mar 1, 1984·Applied and Environmental Microbiology·M A MurryJ R Benemann
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R B Peterson, C P Wolk
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P J Lammers, R Haselkorn

❮ Previous
Next ❯

Citations

Jan 1, 1996·Annual Review of Genetics·C P Wolk
Mar 3, 2009·Journal of Basic Microbiology·Kerstin NicolaisenEnrico Schleiff
Nov 8, 1996·The Journal of Biological Chemistry·W J Keenleyside, C Whitfield
Oct 1, 1995·European Journal of Biochemistry·O SchmitzH Bothe
Sep 18, 2004·FEMS Microbiology Reviews·Antonia HerreroEnrique Flores
Jan 1, 2014·Journal of Bacteriology·Patrick VideauSean M Callahan
Jun 19, 2019·Journal of Bacteriology·Wei-Yue Xing, Cheng-Cai Zhang
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·John C Meeks, Jeff Elhai
Sep 18, 2004·Journal of Bacteriology·Xiaoqiang WuJames W Golden
Mar 19, 2005·Journal of Bacteriology·Martha E RamírezStephanie E Curtis
Jul 1, 1996·Journal of Bacteriology·M I Muro-PastorF J Florencio

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.