Identification and characterization of human embryonic poly(A) binding protein (EPAB)

Molecular Human Reproduction
Ozlem Guzeloglu-KayisliEmre Seli

Abstract

Transcriptional silencing that begins with oocyte maturation persists during the initial mitotic divisions of the embryo. Gene expression during this period largely depends on the translational activation of maternal mRNAs by cytoplasmic polyadenylation and requires an embryonic poly(A) binding protein (EPAB). EPAB has been identified in Xenopus and mouse, where it is expressed exclusively in oocytes and early embryos until zygotic genome activation (ZGA) when it is replaced by the somatic cytoplasmic poly(A) binding protein (PABPC1). EPAB plays a central role in the regulation of maternal mRNA activation by preventing deadenylation and promoting translation. In this study, we identified and characterized the human EPAB ortholog. Human EPAB is a 619 amino acid protein with 77% identity and 84% similarity to mouse EPAB. Human EPAB mRNA is detected in ovaries, testes and several somatic tissues including pancreas, liver and thymus. Similar to the observations in Xenopus and mouse, human EPAB is the predominant poly(A) binding protein in immature (germinal vesicle) and mature (metaphase II) oocytes, and it is replaced by PABPC1 following ZGA, which occurs at 4- to 8-cell stage in human. Our findings suggest that the unique transla...Continue Reading

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Citations

May 27, 2009·Current Opinion in Obstetrics & Gynecology·Martine VercammenHilde Van de Velde
Nov 6, 2014·Journal of Assisted Reproduction and Genetics·Saffet OzturkNecdet Demir
Nov 26, 2015·Wiley Interdisciplinary Reviews. RNA·Juan M Reyes, Pablo J Ross
Mar 19, 2013·Fertility and Sterility·Asli UyarEmre Seli
Nov 27, 2015·Biological Reviews of the Cambridge Philosophical Society·Eline WydoogheAnn Van Soom
Jun 19, 2013·Wiley Interdisciplinary Reviews. RNA·Amanda CharlesworthCornelia H de Moor
May 3, 2014·Wiley Interdisciplinary Reviews. RNA·Callie P WigingtonAnita H Corbett
Aug 22, 2009·Molecular Reproduction and Development·Corinna SiemerWolfgang Tomek
May 25, 2012·The Biochemical Journal·Ozlem Guzeloglu-KayisliEmre Seli
Jun 10, 2016·Human Reproduction Update·Elisabetta Tosti, Yves Ménézo
Mar 5, 2017·Theriogenology·Léonie Morin-DoréMarc-André Sirard
Jul 21, 2012·Reproductive Sciences·Saffet OzturkEmre Seli
May 22, 2018·The Journal of Reproduction and Development·Saffet Ozturk, Fatma Uysal
Jan 20, 2017·Reproduction, Fertility, and Development·Saffet Ozturk, Fatma Uysal
Aug 9, 2018·Frontiers in Physiology·Kat S Moore, Marieke von Lindern

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