Identification and characterization of immune-related microRNAs in blunt snout bream, Megalobrama amblycephala

Fish & Shellfish Immunology
Jiang YuhongYan Jinpeng

Abstract

MicroRNAs (miRNAs) play vital roles in diverse biological processes, including in immune response. Blunt snout bream (Megalobrama amblycephala) is a prevalent and important commercial endemic freshwater fish species in China's intensive polyculture systems. To identify immune-related miRNAs of M. amblycephala, two small RNA (sRNA) libraries from immune tissues with or without lipopolysaccharide (LPS) stimulation were constructed and sequenced using the high-throughput sequencing technology. Totally, 16,425,543 and 15,076,813 raw reads, corresponding to 14,156,755 and 13,445,869 clean reads, were obtained in the normal and infected libraries, respectively. A total of 324 miRNAs, including 218 known miRNAs and 106 putative novel miRNAs were identified by bioinformatic analysis. We analyzed differentially expressed miRNAs between two libraries using pairwise comparison. 113 (34.88%) miRNAs were found to be significantly differentially expressed between two libraries, with 63 (55.75%) exhibiting elevated expression in LPS stimulation sample. Thereinto, a number of known miRNAs were identified immune-related. Real-time quantitative PCR (RT-qPCR) were implemented for 12 miRNAs of two samples, and agreement was confirmed between the s...Continue Reading

References

Oct 3, 2001·Journal of Endotoxin Research·C Alexander, E T Rietschel
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 20, 2003·RNA·Victor AmbrosThomas Tuschl
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Sep 17, 2004·Nature·Victor Ambros
Sep 17, 2004·The EMBO Journal·Yoontae LeeV Narry Kim
Oct 27, 2004·PLoS Biology·Bino JohnDebora S Marks
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
Mar 21, 2007·Trends in Genetics : TIG·Timothy W Nilsen
May 18, 2007·Annual Review of Cell and Developmental Biology·Natascha Bushati, Stephen M Cohen
Apr 9, 2008·International Journal of Medical Sciences·Esmerina TiliGeorge Adrian Calin
Apr 1, 2009·Briefings in Bioinformatics·Chad J CreightonPreethi H Gunaratne
May 20, 2009·Genes & Development·Jennifer L Umbach, Bryan R Cullen
Jan 26, 2010·Nature Reviews. Immunology·Ryan M O'ConnellDavid Baltimore
May 26, 2010·International Immunology·Murali GururajanMax D Cooper
Sep 14, 2011·Nucleic Acids Research·Marc R FriedländerNikolaus Rajewsky
Jun 6, 2012·RNA Biology·Ana EulalioJörg Vogel
Jun 19, 2013·Journal of Internal Medicine·M HaneklausS L Masters
Nov 28, 2013·Nucleic Acids Research·Ana Kozomara, Sam Griffiths-Jones
Mar 22, 2014·Biochemical Society Transactions·Divya Vashisht, Michael D Nodine

❮ Previous
Next ❯

Citations

Dec 14, 2018·Frontiers in Immunology·Man WangKun Wang
Sep 14, 2018·Asian-Australasian Journal of Animal Sciences·Jingge LiuHonglin Liu
Feb 21, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yan-Ling SunLi Sun
Mar 27, 2021·Fish & Shellfish Immunology·Wei ZhouChao Ran
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Yun ZhaoXu Zhou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.