Identification and characterization of Inositol-requiring enzyme-1 and X-box binding protein 1, two proteins involved in the unfolded protein response of Litopenaeus vannamei

Developmental and Comparative Immunology
Yi-Hong ChenJian-Guo He

Abstract

The inositol-requiring enzyme-1 (IRE1)-X-box binding protein 1 (IRE1-XBP1) pathway is the key branch of the unfolded protein response (UPR). To investigate the role of the IRE1-XBP1 pathway in reducing environmental stress and increasing anti-viral immunity in Litopenaeus vannamei, homologues of IRE1 (designated as LvIRE1) and XBP1 (designated as LvXBP1) were identified and characterized. The full-length cDNA of LvIRE1 is 4908bp long, with an open reading frame (ORF) that encodies a putative 1174 amino acid protein. The full-length cDNA of LvXBP1 is 1746bp long. It contains two ORFs that encode putative 278 amino acid and 157 amino acid proteins, respectively. LvXBP1 mRNA has the predicted IRE1 splicing motifs CNG'CNGN located within the loop regions of two short hairpins. Sequencing of the splicing fragment induced by endoplasmic reticulum (ER)-stress showed a 3bp or 4bp frame shift from the predicted sites. The spliced form LvXBP1 (LvXBP1s) contained an ORF encodes a putative 463 amino acid protein. The reporter gene assays indicated that LvXBP1s activates the promoter of L. vannamei immunoglobulin heavy chain binding protein (LvBip), an important UPR effector. RT-PCR showed that LvXBP1 was spliced during the experiments. For...Continue Reading

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Citations

Dec 23, 2015·Developmental and Comparative Immunology·Yong-Gui ChenYi-Hong Chen
Dec 21, 2014·Fish & Shellfish Immunology·Hongliang ZuoXiaopeng Xu
Aug 26, 2014·Fish & Shellfish Immunology·Jingxiang XuHong Shi
Oct 27, 2015·Fish & Shellfish Immunology·Yi-Hong ChenJian-Guo He
Dec 4, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Zhenlu WangLanfen Fan

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