PMID: 9160167Apr 1, 1997Paper

Identification and characterization of inositol 1,4,5-trisphosphate receptors in rat testis

Cell Calcium
S C ToveyF Michelangeli

Abstract

PCR analysis and immunoblotting with isoform specific antibodies was used to identify the presence of type I, II and III inositol 1,4,5-trisphosphate receptors (InsP3Rs) in rat testis. PCR analysis also revealed that rat testis express both forms of the S1 splice variant (S1+ and S1-), but only the S2- from of the S2 splice variant of the type I InsP3 receptor. PCR analysis was also used to identify InsP3R isoform expression at a cellular level using myoid, Sertoli and germ cells derived from the testis of Wistar rats. The extent of [3H]-InsP3 binding was found to be 9 times lower for testicular microsomes than for cerebellar microsomes, with a Bmax of 1.4 pmoles/mg protein compared to 12.5 pmoles/mg protein for cerebellar microsomes. The Kd for InsP3 binding to its receptor in testicular microsomes was 60 +/- 10 nM which was similar to that found for cerebellar microsomes (80 +/- 20 nM). InsP3-induced Ca2+ release (IICR) in testicular microsomes was found to have an EC50 (concentration which causes a half-maximal response) of 0.5 +/- 0.03 microM, also similar to that seen for cerebellar microsomes (0.3 microM). Maximal IICR occurred at about 20 microM InsP3, with up to 4% of total intracellular Ca2+ stores being mobilized as c...Continue Reading

References

Oct 1, 1992·The International Journal of Biochemistry·P VolpeB H Alderson-Lang
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C A RossA Ullrich
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T NakagawaK Mikoshiba
Jan 1, 1991·Pharmacology & Therapeutics·C W Taylor, A Richardson
Jan 1, 1991·Hormone Research·J M SaezP G Chatelain
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S K DanoffC A Ross
Feb 1, 1991·Endocrine Reviews·M K Skinner
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T Meyer, L Stryer
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Aug 14, 1987·Biochemical and Biophysical Research Communications·A L WillcocksS R Nahorski
Jan 1, 1988·Annual Review of Physiology·M L Dufau
Jan 1, 1985·Analytical Biochemistry·J M GershoniG E Palade
Aug 1, 1970·The Biochemical Journal·D E Brooks
Nov 15, 1995·The Biochemical Journal·M J Berridge
Jan 28, 1993·Nature·M J Berridge
Mar 1, 1993·Trends in Pharmacological Sciences·K Mikoshiba
Sep 1, 1995·Neuropharmacology·F MichelangeliL G Sayers
Aug 1, 1995·Molecular and Cellular Biochemistry·S A RudgeC J Kirk

❮ Previous
Next ❯

Citations

Oct 3, 2003·Biochemical and Biophysical Research Communications·Shahla Zafar KhanFrancesco Michelangeli
Oct 1, 1998·European Journal of Pharmacology·S C ToveyF Michelangeli
Jun 22, 2000·The Biochemical Journal·F X BoittinJ Mironneau
Nov 4, 2000·Biochemical and Biophysical Research Communications·P J HughesF Michelangeli
Mar 11, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Oluseye A OgunbayoFrancesco Michelangeli
Aug 26, 2004·Biochimica Et Biophysica Acta·Laura L WoottonFrancesco Michelangeli
Nov 4, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·S C ToveyF Michelangeli
Dec 13, 2005·Human Reproduction Update·C Jimenez-GonzalezS J Publicover

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.