Identification and Characterization of l-Malate Dehydrogenases and the l-Lactate-Biosynthetic Pathway in Leuconostoc mesenteroides ATCC 8293

Journal of Agricultural and Food Chemistry
Kyung Hyun KimChe Ok Jeon

Abstract

One putative l-lactate dehydrogenase gene (l- ldh) and three putative d- ldh genes from Leuconostoc mesenteroides ATCC 8293 were overexpressed, and their enzymatic properties were investigated. Only one gene showed d-LDH activity, catalyzing pyruvate and d-lactate interconversion, whereas the other genes displayed l- and d-malate dehydrogenase (MDH) activity, catalyzing oxaloacetate and l- and d-malate interconversion, suggesting that strain ATCC 8293 may not harbor an l- ldh gene. Putative phosphoenolpyruvate carboxylase (PEPC)- and malolactic enzyme (MLE)-encoding genes were identified from strain ATCC 8293, and sequence analysis showed that they could exhibit PEPC and MLE activities, respectively. l-Lactate production and transcriptional expression of the mle gene in this strain were highly increased in the presence of l-malate. We propose that in strain ATCC 8293, which lacks an l- ldh gene, l-lactate is produced through sequential enzymatic conversions from phosphoenolpyruvate to oxaloacetate, then l-malate, and finally l-lactate by PEPC, l-MDH, and MLE, respectively.

References

Aug 1, 1991·Applied and Environmental Microbiology·S F KimM Y Pack
Mar 1, 1980·Microbiological Reviews·E I Garvie
Mar 1, 1995·Canadian Journal of Microbiology·A Oren, P Gurevich
Feb 26, 2000·Enzyme and Microbial Technology·K Hofvendahl, B Hahn-Hägerdal
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 25, 2002·Journal of Molecular Evolution·Dominique Madern
Jun 11, 2002·Journal of Molecular Biology·Adelia RazetoVictor S Lamzin
Dec 27, 2002·The Journal of General and Applied Microbiology·Akira ManomeKazuo Komagata
Feb 1, 1982·Applied and Environmental Microbiology·A Lonvaud-Funel, A M de Saad
Jan 1, 1997·Applied and Environmental Microbiology·C FoucaudJ Richard
Jun 26, 2007·Applied and Environmental Microbiology·Minjeong ParkChe Ok Jeon
Feb 15, 2011·Applied and Environmental Microbiology·Ji Young JungChe Ok Jeon
Dec 23, 2011·International Journal of Food Microbiology·Ji Young JungChe Ok Jeon
Nov 6, 2012·Food Microbiology·Raffaella Di CagnoMarco Gobbetti
Jan 15, 2014·Applied Microbiology and Biotechnology·Ji Young JungChe Ok Jeon
Oct 29, 2014·Nucleic Acids Research·UNKNOWN UniProt Consortium
May 16, 2015·Gastroenterology Research and Practice·N Gurukripa Kowlgi, Lovely Chhabra
Dec 16, 2016·Journal of Agricultural and Food Chemistry·Holger Zorn, Qing X Li
Aug 24, 2017·FEMS Microbiology Reviews·Benoît DesguinPascal Hols

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Citations

Aug 5, 2020·Applied Microbiology and Biotechnology·Se Hee LeeChe Ok Jeon
Jul 16, 2020·Applied Microbiology and Biotechnology·Juliana BleckwedelRaúl Ricardo Raya
Jul 21, 2020·BioMed Research International·Miroslav Pohanka

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