Identification and characterization of microRNAs in the intestinal tissues of sheep (Ovis aries)

PloS One
Lei HouJianmin Wang

Abstract

Sheep are small ruminants, and their long intestines exhibit high digestive and absorptive capacity in many different rearing conditions; however, the genetic bases of this characteristic remains unclear. MicroRNAs (miRNAs) play a major role in maintaining both intestinal morphological structure as well as in regulating the physiological functions of this organ. However, no study has reported on the miRNA expression profile in the intestinal tissues of sheep. Here, we analyzed and identified the miRNA expression profile of three different intestinal tissues (i.e., duodenum, cecum, and colon) of sheep (Ovis aries) using high-throughput sequencing and bioinformatic methods. In total, 106 known miRNAs were identified, 458 conserved miRNAs were detected, 192 unannotated novel miRNAs were predicted, and 195 differentially expressed miRNAs were found between the different tissues. Additionally, 3,437 candidate target genes were predicted, and 17 non-redundant significantly enriched GO terms were identified using enrichment analysis. A total of 99 candidate target genes were found to significantly enriched in 4 KEGG biological pathways. A combined regulatory network was constructed based on 92 metabolism-related candidate target genes...Continue Reading

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Citations

Feb 26, 2020·Animals : an Open Access Journal From MDPI·Suyu QuanBenhai Xiong
Jun 6, 2020·Scientific Reports·Clara Yieh Lin ChongJustin M O'Sullivan
Mar 4, 2020·Animals : an Open Access Journal From MDPI·Yaseen UllahShengwei Hu
Jan 10, 2021·Trends in Microbiology·Nilusha Malmuthuge, Le Luo Guan
Oct 30, 2020·International Journal of Molecular Sciences·Ankita SharmaNiel A Karrow
Jul 23, 2021·Animal : an International Journal of Animal Bioscience·Y ZhangS Liang

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Methods Mentioned

BETA
PCR
RNAseq

Software Mentioned

Targetscan
miRanda
SOAP aligner
DAVID
miReap
Cytoscape
BLAST
STRING

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