PMID: 7527084Jan 1, 1995Paper

Identification and characterization of monoclonal antibodies specific for polymorphic antigenic determinants within the V2 region of the human immunodeficiency virus type 1 envelope glycoprotein

Journal of Virology
C ShottonJ A McKeating

Abstract

We have identified six monoclonal antibodies (MAbs) mapping to both linear and conformation-dependent epitopes within the V2 region of the human immunodeficiency virus type 1 clone HXB10. Three of the MAbs (12b, 66c, and 66a) were able to neutralize the molecular clones HXB10 and HXB2, with titers in the range of 9.5 to 20.0 micrograms/ml. MAbs mapping to the crown of the V2 loop (12b, 60b, and 74) bound poorly to cell surface-expressed oligomeric gp120, suggesting an explanation for the poor or negligible neutralizing activity of MAbs to this region. In contrast, MAbs 12b and 60b demonstrated good reactivity with recombinant gp120 in an enzyme-linked immunosorbent assay format, suggesting differential epitope exposure between the recombinant and native forms of gp120. Cross-competition analysis of these MAbs and additional V1V2 MAbs for gp120 binding enabled us to assign the MAbs to six groups (A to F). Selection of neutralization escape mutants with MAbs 10/76b and 11/68b, belonging to nonoverlapping competition groups, identified amino acid changes at residues 165 (I to T) and 185 (D to N), respectively. Interestingly, these escape variants remained sensitive to neutralization by the nonselecting V2 MAbs. All MAbs demonstrat...Continue Reading

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T ShiodaC Cheng-Mayer
Apr 1, 1992·Journal of Virology·P WesterveltL Ratner
Aug 1, 1991·The Journal of Experimental Medicine·Q J Sattentau, J P Moore
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D D HoN C Sun
Jan 1, 1991·AIDS·J P Moore, P L Nara
Jun 4, 1993·Science·M GroeninkH Schuitemaker

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Citations

Mar 23, 2006·Annual Review of Immunology·Ralph Pantophlet, Dennis R Burton
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuxian HeAbraham Pinter
Jul 23, 2014·AIDS Research and Human Retroviruses·Evgeny ShmelkovTimothy Cardozo
Jan 6, 2010·Current Opinion in HIV and AIDS·William R SchiefLeonidas Stamatatos
Apr 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T J HewsonS E Howie
Feb 1, 2012·AIDS Research and Human Retroviruses·Silawun AmpolWannee Kantakamalakul
Nov 21, 1997·Journal of Molecular Biology·N W DouglasR S Daniels
Sep 1, 1995·Trends in Microbiology·A McKnight, P R Clapham
Mar 5, 1999·Immunology Letters·J A McKeating, P Balfe

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