Identification and characterization of Myosin from rat testicular peritubular myoid cells.

Biology of Reproduction
Dario FernándezLuis A Lopez

Abstract

In the mammalian testis, peritubular myoid cells (PMCs) surround seminiferous tubules. These cells are contractile, express the cytoskeletal markers of true smooth muscle-alpha-isoactin and F-actin-and participate in the contraction of seminiferous tubules during the transport of spermatozoa and testicular fluid to the rete testis. Myosin from PMCs (PMC-myosin) was isolated from adult rat testis and purified by cycles of assembly-disassembly and sucrose gradient centrifugation. PMC-myosin was recognized by a monoclonal anti-smooth muscle myosin antibody, and the peptide sequence shared partial homology with rat smooth muscle myosin-II, MYH11 (also known as SMM-II). Most PMC-myosin (95%) was soluble in the PMC cytosol, and purified PMC-myosin did not assemble into filaments in the in vitro salt dialysis assay at 4 degrees C, but did at 20 degrees C. PMC-myosin filaments are stable to ionic strength to the same degree as gizzard MYH11 filaments, but PMC-myosin filaments were more unstable in the presence of ATP. When PMCs were induced to contract by endothelin 1, a fraction of the PMC-myosin was found to be involved in the contraction. From these results we infer that PMCs express an isoform of smooth muscle myosin-II that is cha...Continue Reading

References

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Citations

Sep 22, 2009·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Martin Albrecht
Mar 19, 2016·Cell and Tissue Research·Antonella D LosinnoAlfonsina Morales
Jun 2, 2017·Scientific Reports·Nathália L M LaraRichard M Sharpe
Feb 12, 2021·Endocrinology·Nathalia de Lima E Martins LaraIna Dobrinski

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Datasets Mentioned

BETA
MYH11

Methods Mentioned

BETA
Electrophoresis
electrophoreses
scanning electron microscopy
light microscopy

Software Mentioned

ImageJ
Protein Lynx Global Server
Mascot Server
Scion Image
MassLynx

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