Identification and characterization of nematode specific protective epitopes of Brugia malayi TRX towards development of synthetic vaccine construct for lymphatic filariasis

Vaccine
Jayaprakasam MadhumathiPerumal Kaliraj

Abstract

Although multi-epitope vaccines have been evaluated for various diseases, they have not yet been investigated for lymphatic filariasis. Here, we report for the first time identification of two immunodominant B epitopes (TRXP1 and TRXP2) from the antioxidant Brugia malayi thioredoxin by studying their immune responses in mice model and human subjects. TRXP1 was also found to harbor a T epitope recognized by human PBMCs and mice splenocytes. Further, the epitopic peptides were synthesized as a single peptide conjugate (PC1) and their prophylactic efficacy was tested in a murine model of filariasis with L3 larvae. PC1 conferred a significantly high protection (75.14%) (P < 0.0001) compared to control (3.7%) and recombinant TRX (63.03%) (P < 0.018) in experimental filariasis. Our results suggest that multi-epitope vaccines could be a promising strategy in the control of lymphatic filariasis.

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Citations

Jul 5, 2013·Clinical Microbiology Reviews·C Paul MorrisEdward Mitre
Oct 23, 2013·PloS One·Gajalakshmi DakshinamoorthyRamaswamy Kalyanasundaram
Jul 17, 2012·Veterinary Immunology and Immunopathology·Satya Narayan PradhanUsha Antony
Jul 22, 2014·Scandinavian Journal of Immunology·N MahalakshmiP Kaliraj
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Oct 6, 2012·Vaccine·Gajalakshmi DakshinamoorthyRamaswamy Kalyanasundaram
Aug 15, 2014·Acta Parasitologica·Jeya Prita Parasurama JawaharlalPerumal Kaliraj
Jan 13, 2015·International Immunopharmacology·Jeya Prita Parasurama JawaharlalKaliraj Perumal
Nov 30, 2014·International Journal of Biological Macromolecules·Balasubramaniyan MalathiPerumal Kaliraj
Jul 17, 2015·Scandinavian Journal of Immunology·G AnugrahaP Kaliraj
Apr 23, 2017·Immunologic Research·Christiana ImmanuelKaliraj Perumal
Sep 16, 2019·International Journal of Biological Macromolecules·Nikhilesh Joardar, Santi P Sinha Babu
Oct 12, 2021·Expert Review of Vaccines·Vivek P ChavdaVandana Patravale

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