Identification and characterization of novel ERC-55 interacting proteins: evidence for the existence of several ERC-55 splicing variants; including the cytosolic ERC-55-C

Proteomics
Maja LudvigsenB Honoré

Abstract

ERC-55, encoded from RCN2, is localized in the ER and belongs to the CREC protein family. ERC-55 is involved in various diseases and abnormal cell behavior, however, the function is not well defined and it has controversially been reported to interact with a cytosolic protein, the vitamin D receptor. We have used a number of proteomic techniques to further our functional understanding of ERC-55. By affinity purification, we observed interaction with a large variety of proteins, including those secreted and localized outside of the secretory pathway, in the cytosol and also in various organelles. We confirm the existence of several ERC-55 splicing variants including ERC-55-C localized in the cytosol in association with the cytoskeleton. Localization was verified by immunoelectron microscopy and sub-cellular fractionation. Interaction of lactoferrin, S100P, calcyclin (S100A6), peroxiredoxin-6, kininogen and lysozyme with ERC-55 was further studied in vitro by SPR experiments. Interaction of S100P requires [Ca(2+)] of approximately 10(-7) M or greater, while calcyclin interaction requires [Ca(2+)] of >10(-5) M. Interaction with peroxiredoxin-6 is independent of Ca(2+). Co-localization of lactoferrin, S100P and calcyclin with ERC-5...Continue Reading

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Citations

Jun 15, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Ani ManichaikulWeibin Shi
Dec 1, 2012·Basic Research in Cardiology·Massimiliano ManciniPaolo G Camici
Oct 23, 2012·Journal of Cellular Physiology·Ahmed A RehmanFahim H Khan
Apr 4, 2019·American Journal of Physiology. Renal Physiology·Jing LiWeibin Shi
Mar 28, 2017·Biological Chemistry·Wiesława LeśniakAnna Filipek
Aug 30, 2018·Skeletal Muscle·José Andrés González CoraspeAndreas Roos
Oct 1, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christopher J PanebiancoJames C Iatridis

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