Identification and characterization of novel senescence-associated genes from barley (Hordeum vulgare) primary leaves

Plant Biology
N AyKlaus Humbeck

Abstract

Leaf senescence is the final developmental stage of a leaf. The progression of barley primary leaf senescence was followed by measuring the senescence-specific decrease in chlorophyll content and photosystem II efficiency. In order to isolate novel factors involved in leaf senescence, a differential display approach with mRNA populations from young and senescing primary barley leaves was applied. In this approach, 90 senescence up-regulated cDNAs were identified. Nine of these clones were, after sequence analyses, further characterized. The senescence-associated expression was confirmed by Northern analyses or quantitative RealTime-PCR. In addition, involvement of the phytohormones ethylene and abscisic acid in regulation of these nine novel senescence-induced cDNA fragments was investigated. Two cDNA clones showed homologies to genes with a putative regulatory function. Two clones possessed high homologies to barley retroelements, and five clones may be involved in degradation or transport processes. One of these genes was further analysed. It encodes an ADP ribosylation factor 1-like protein (HvARF1) and includes sequence motifs representing a myristoylation site and four typical and well conserved ARF-like protein domains. T...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Aug 1, 1994·Current Opinion in Cell Biology·J G Donaldson, R D Klausner
Apr 1, 1997·Current Opinion in Biotechnology·H G Nam
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jul 1, 1997·The Plant Cell·A B Bleecker, S E Patterson
May 23, 1998·The Journal of Cell Biology·M PassreiterW W Just
Aug 26, 1998·Biochimica Et Biophysica Acta·P Dupree, D J Sherrier
Sep 17, 1999·The Plant Cell·C M VicientA H Schulman
Apr 5, 2000·Biochimica Et Biophysica Acta·A Ortiz-LopezD R Bush
Jun 29, 2000·Trends in Plant Science·B F QuirinoR M Amasino
Jun 30, 2000·Current Opinion in Cell Biology·J G Donaldson, C L Jackson
Jul 4, 2001·Journal of Experimental Botany·C F JohnS A-H-Mackerness
Oct 18, 2001·Journal of Experimental Botany·L D Noodén, J P Penney
May 10, 2002·Genes & Development·Silke Robatzek, Imre E Somssich
May 22, 2002·Functional & Integrative Genomics·Nils RostoksAndris Kleinhofs
Feb 28, 2003·Journal of Plant Research·Jing CaoKeming Cui
Sep 11, 2003·Journal of Plant Physiology·Kuo Tung Hung, Ching Huei Kao
Sep 12, 2003·Developmental Cell·Zhongzhen NiePaul A Randazzo

❮ Previous
Next ❯

Citations

Apr 30, 2013·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hai-Bin WuLetian Chen
May 18, 2012·The New Phytologist·Rongli ShiNicolaus von Wirén
Aug 3, 2014·Biochimica Et Biophysica Acta·Marie-Angèle Grandbastien
Oct 26, 2016·Frontiers in Plant Science·Pooja NegiPenna Suprasanna
Apr 1, 2014·Journal of Experimental Botany·Nicole AyKlaus Humbeck
Sep 20, 2008·Plant Biology·K Krupinska, K Humbeck
Jul 24, 2020·International Journal of Molecular Sciences·Agnieszka Ostrowska-MazurekEwa Sobieszczuk-Nowicka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.