Identification and characterization of novel phosphate regulon genes, ecs0540-ecs0544, in Escherichia coli O157:H7.

Molecular Genetics and Genomics : MGG
Yusuke YoshidaKozo Makino

Abstract

In response to environmental phosphate limitation, the transcriptional activator PhoB of Escherichia coli (E. coli) activates transcription of the phosphate regulon (pho regulon) genes that are involved in phosphate utilization. At least 31 of pho regulon genes have been identified and well characterized in E. coli by numerous studies using non-pathogenic K-12 derivative strains. In this study, we searched for PhoB-regulated promoters from a lacZ-fused genomic library of the E. coli O157:H7 Sakai in an attempt to find novel pho regulon genes in the strain. A promoter region located upstream of a gene cluster (ecs0540-ecs0544) that mapped within one of the strain-specific chromosomal regions of the E. coli O157:H7 was identified. By further in vivo analysis with various subclones of the 5'-flanking region, it was suggested that the ecs0540 transcription was regulated by at least two promoters, an upstream PhoB-regulated promoter and a downstream constitutive promoter. S1 mapping and footprinting experiments revealed two transcription start sites and a sequence similar to the consensus sequence of PhoB binding, respectively. Bioinformatic analysis of the ecs0540-ecs0544 genes showed that these genes were highly homologous to the ...Continue Reading

References

Oct 1, 1987·Journal of Bacteriology·S B Calderwood, J J Mekalanos
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jul 5, 1982·Journal of Molecular Biology·B L Wanner, R McSharry
May 1, 1993·Journal of Bacteriology·A Ishihama
May 31, 1996·Journal of Molecular Biology·K MakinoM Suzuki
Sep 2, 1999·Trends in Microbiology·E T LallyJ Korostoff
Mar 22, 2001·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T HayashiH Shinagawa
Feb 13, 2003·The Journal of Biological Chemistry·Aitziber L CortajarenaHelena Ostolaza
Feb 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wanyin DengB Brett Finlay
Nov 4, 2004·Molecular Microbiology·Eirwen MorganTimothy S Wallis
May 30, 2006·Current Microbiology·Narelle FeganKari Steven Gobius
Jun 13, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Yoshitoshi OguraTetsuya Hayashi
Feb 8, 2008·Journal of Clinical Microbiology·Vincenzo SaviniDomenico D'Antonio
Feb 23, 2010·Current Opinion in Microbiology·Yi-Ju Hsieh, Barry L Wanner

❮ Previous
Next ❯

Citations

May 4, 2011·The Journal of Microbiology·Yusuke YoshidaKozo Makino
Jul 7, 2011·Genetics and Molecular Research : GMR·S C SoaresA Miyoshi
May 23, 2014·Journal of Proteomics·Vanessa Rodrigues PegosAndrea Balan
Jun 28, 2011·Veterinary Microbiology·Sébastien CrépinJosée Harel
Oct 3, 2012·Metabolic Engineering·Kevin V SolomonKristala L J Prather
Sep 15, 2019·EcoSal Plus·Stewart G Gardner, William R McCleary
Mar 9, 2021·Environmental Microbiology·Holly ShropshireYin Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.