PMID: 8946055Nov 22, 1996Paper

Identification and characterization of novel developmentally regulated proteins in rat spinal cord

Brain Research. Developmental Brain Research
D H GeschwindS Hockfield

Abstract

We previously used 2-dimensional (2-D) gel electrophoresis to identify novel proteins that may be involved in the genesis of the mammalian nervous system [1]. Several novel proteins that were up- or down-regulated coincident with neurogenesis and neuronal migration in rat neocortex were identified. To further investigate the expression of some of these developmentally regulated proteins during a comparable period in spinal cord development, 2-D electrophoresis is used to study their regulation in the crude membrane and soluble fractions of spinal cord at embryonic day 12 (E12) and embryonic day 21 (E21). This analysis indicates that 7 of the proteins that exhibited large changes in their synthesis in cerebral cortex between embryonic day 14 (E14) and embryonic day 21 (E21) demonstrate similar up- or down-regulation during spinal cord neurogenesis. However, two proteins are restricted in their expression or developmental regulation. One of these, 667-800, appears cortex-specific, while the up-regulation of protein SC.1 appears to be spinal cord specific. Several of these proteins also appear to be enriched in both the cortex and spinal cord relative to non-neural tissues (117, 162, 182, 310 [TOAD-64], 667-800) and may be neural ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 10, 1976·Biochemistry·G F Ames, K Nikaido
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Feb 22, 1991·The Journal of Comparative Neurology·S D WangM Murray
Jan 1, 1991·Neuroscience·M FitzgeraldL I Benowitz
Jan 1, 1991·Annual Review of Neuroscience·S K McConnell
Jan 1, 1987·Current Topics in Developmental Biology·B G Szaro, Y P Loh
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R L NeveL I Benowitz
Jun 1, 1986·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M JacewiczW A Pulsinelli
Jan 1, 1984·Experimental Neurology·J P Cummings, D J Stelzner
Feb 15, 1984·The Biochemical Journal·S F Leong, J B Clark
Feb 15, 1984·The Biochemical Journal·S F Leong, J B Clark
May 8, 1995·The Journal of Comparative Neurology·J E MinturnS Hockfield
May 1, 1995·Journal of Neurochemistry·F R ThormodssonS Hockfield
Jan 1, 1993·Journal of Neurocytology·R CurtisG P Wilkin
May 1, 1981·Surgical Neurology·D Ruge

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Citations

Mar 5, 1999·Brain Research. Developmental Brain Research·C D AlbrightS H Zeisel
Jul 22, 2006·Journal of Neurobiology·Andrea Megela SimmonsRebecca A Brown
Jul 24, 2012·Journal of Anatomy·Atsuhiro Tsutiya, Ritsuko Ohtani-Kaneko
Oct 10, 2002·The Journal of Comparative Neurology·Anabel FernándezOmar Trujillo-Cenóz
May 11, 2010·Neuroreport·Kaya BorkRüdiger Horstkorte

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