Feb 9, 2016

Identification and characterization of novel Bovine Leukemia Virus (BLV) antisense transcripts reveals their constitutive expression in leukemic and pre-leukemic clones

BioRxiv : the Preprint Server for Biology
Keith DurkinAnne Van den Broeke


Bovine Leukemia Virus (BLV) is a deltaretrovirus closely related to the Human T-cell leukemia virus-1 (HTLV-1). Cattle are the natural host of BLV where it integrates into B-cells and produces a lifelong infection. Most infected animals remain asymptomatic but following a protracted latency period about ~5% develop an aggressive leukemia/lymphoma, mirroring the disease trajectory of HTLV-1. The 5′LTRs of both the BLV and HTLV-1 proviruses are transcriptionally silent in tumors, however they are not entirely quiescent, with the HLTV-1 antisense transcript HBZ and the BLV microRNAs constitutively expressed in tumors. Here, using RNA-seq, we demonstrate that in addition to microRNAs, the BLV provirus also constitutively expresses two antisense transcripts in all BLV infected samples examined. The first transcript (AS1) has alternate potential polyadenylation sites generating a short transcript of ~600bp (AS1-S) and a less abundant longer transcript of ~2200bp (AS1-L). Alternative splicing also creates a second transcript of ~400bp (AS2) utilizing the first exon of AS1. Production of AS transcripts from the 3′LTR was supported by reporter assays demonstrating that the BLV LTR has substantial and Tax-independent antisense promoter a...Continue Reading

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Mentioned in this Paper

Antineoplaston AS 2-1
Gene Knockdown Techniques
BLV Infections
RNA Ligase (ATP)
SCPEP1 gene
Long Terminal Repeat
Sequence Determinations, RNA
Leukemia Virus Antibodies, Human T-Cell
Transcription, Genetic

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