Identification and characterization of porcine mannan-binding lectin A (pMBL-A), and determination of serum concentration heritability

Immunogenetics
Helle R Juul-MadsenUffe Holmskov

Abstract

Mannan-binding lectin (MBL) is an innate immune collectin present in the serum of humans and many farm animals. This oligomeric pattern-recognition protein effectively binds to the glycoconjugate arrays present on the surfaces of microorganisms and activates the complement system to enhance pathogen killing and clearance. MBL deficiency is often associated with immunodeficiency in humans. Although two MBLs (MBL-A and MBL-C) have been characterized in various species, the identity of porcine MBL (pMBL) was not clearly defined. In this study, we purified an MBL from porcine serum by mannose affinity, ion exchange, and size exclusion chromatography and determined many of its characteristics. Based on the N-terminal sequence, multiple sequence alignment, and relative affinities to various carbohydrate ligands, we propose that the MBL purified in this study is pMBL-A. We have generated antibodies to this protein and established an immunoassay to quantify pMBL-A in serum. Using this assay, we found breed differences in pMBL-A concentration distributions and heritability estimates. In the Duroc breed (n=588), pMBL-A concentrations show a unimodal distribution with a mean of 9,125 ng/ml. In contrast, the pMBL-A concentration distributi...Continue Reading

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Citations

Nov 8, 2006·Immunogenetics·Brandon N LillieM Anthony Hayes
Nov 10, 2012·Journal of Animal Science and Biotechnology·Shuhong ZhaoHongbo Chen
Aug 25, 2009·Veterinary Immunology and Immunopathology·Helle R Juul-MadsenBirthe M Damgaard
Mar 31, 2009·Molecular Immunology·Bodil Salvesen, Tom E Mollnes
Mar 27, 2007·Fish & Shellfish Immunology·Karrie M YoungJohn S Lumsden
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jun 8, 2012·International Journal of Immunogenetics·I-M BergmanH R Juul-Madsen
Apr 9, 2014·Developmental and Comparative Immunology·K H MairF Meurens
Dec 30, 2006·Developmental and Comparative Immunology·Brandon N LillieM Anthony Hayes
Feb 18, 2011·Upsala Journal of Medical Sciences·Ingrid-Maria Bergman

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