PMID: 9423854Jan 10, 1998Paper

Identification and characterization of protective T cells in hsp65 DNA-vaccinated and Mycobacterium tuberculosis-infected mice

Infection and Immunity
V L BonatoC L Silva

Abstract

Immunization by intramuscular injection of plasmid DNA expressing mycobacterial 65-kDa heat shock protein (hsp65) protects mice against challenge with virulent Mycobacterium tuberculosis H37Rv. During infection or after immunization, CD4+/CD8- and CD8+/CD4- hsp65-reactive T cells increased equally in spleens. During infection, the majority of these cells were weakly CD44 positive (CD44(lo)) and produced interleukin 4 (IL-4) whereas after immunization the majority were highly CD44 positive (CD44(hi)) and produced gamma interferon (IFN-gamma). In adoptive transfer of protection to naive mice, the total CD8+/CD4- cell population purified from spleens of immunized mice was more protective than that from infected mice. When the cells were separated into CD4+/CD8- and CD8+/CD4- types and then into CD44(hi) and CD44(lo) types, CD44(lo) cells were essentially unable to transfer protection, the most protective CD44(hi) cells were CD8+/CD4-, and those from immunized mice were much more protective than those from infected mice. Thus, whereas the CD44(lo) IL-4-producing phenotype prevailed during infection, protection was associated with the CD8+/CD44(hi) IFN-gamma-producing phenotype that predominated after immunization. This conclusion w...Continue Reading

References

Jun 1, 1988·Immunology Today·S H Kaufmann
Jul 1, 1995·Immunology Today·S H Kaufmann
Oct 1, 1995·European Journal of Immunology·C H LadelS H Kaufmann
Jul 1, 1995·The Journal of Clinical Investigation·B Y Lee, M A Horwitz
Aug 1, 1995·Infection and Immunity·M ZhangP F Barnes
Jan 1, 1995·The Journal of Experimental Medicine·R A Seder, G G Le Gros
Jan 1, 1994·Annual Review of Immunology·R A Seder, W E Paul
Aug 1, 1994·Scandinavian Journal of Immunology·B Naume, T Espevik
Aug 1, 1996·Nature Medicine·R E TasconD B Lowrie

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Citations

May 29, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C L SilvaV M Lima
May 3, 2000·The Journal of Clinical Investigation·V A BoussiotisA E Goldfeld
Aug 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Benjamin WizelAlessandro Sette
Jun 4, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·L H FrancoV L D Bonato
Jun 15, 2010·PLoS Neglected Tropical Diseases·Fabiani G FrantzLúcia H Faccioli
Feb 18, 2015·The Journal of Antimicrobial Chemotherapy·Rodrigo F RodriguesCelio L Silva
Sep 24, 2015·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·I C FontouraA A M Coelho-Castelo
Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A WangooD B Young
Jul 27, 1999·Nature·D B LowrieC L Silva
Sep 4, 2003·Human Gene Therapy·Arlete A M Coelho-CasteloCélio Lopes Silva
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R N ColerM R Alderson
Jun 26, 2007·Clinical and Experimental Immunology·R Rodrigues dos Santos JúniorC Lopes Silva
Dec 23, 2008·Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia·Adriana Ignacio de PaduaJosé Antônio Baddini Martinez
Jan 7, 2017·Human Vaccines & Immunotherapeutics·Pryscilla Fanini WowkVânia Luiza Deperon Bonato
Jun 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·N V SerbinaJ L Flynn
Jul 18, 2002·Scandinavian Journal of Immunology·V L D BonatoC L Silva
Sep 21, 2000·Folia Microbiologica·E WeiglS Hradilová
May 18, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Y Riffo-VasquezA Coates
Sep 16, 1998·Clinical and Experimental Immunology·A D Howard, B S Zwilling
Apr 8, 2006·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Milan Raska, Evzen Weigl
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·R TeitelbaumB R Bloom
Oct 31, 2002·Immunology and Cell Biology·Vijaya NagabhushanamChristina Cheers
Mar 24, 2004·Nature Reviews. Microbiology·Graham R StewartDouglas B Young
Nov 2, 2005·Human Gene Therapy·Rubens R Santos-JuniorCélio L Silva
Nov 30, 2013·Clinical & Developmental Immunology·Sofia Fernanda Gonçalves Zorzella-PezaventoAlexandrina Sartori
Nov 19, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D M FonsecaV L D Bonato
Sep 18, 2012·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C D RochaA A M Coelho-Castelo
Mar 21, 2002·Scandinavian Journal of Immunology·V L BonatoC L Silva

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