Identification and characterization of proteins involved in rice urea and arginine catabolism.

Plant Physiology
Feng-Qiu CaoClaus-Peter Witte

Abstract

Rice (Oryza sativa) production relies strongly on nitrogen (N) fertilization with urea, but the proteins involved in rice urea metabolism have not yet been characterized. Coding sequences for rice arginase, urease, and the urease accessory proteins D (UreD), F (UreF), and G (UreG) involved in urease activation were identified and cloned. The functionality of urease and the urease accessory proteins was demonstrated by complementing corresponding Arabidopsis (Arabidopsis thaliana) mutants and by multiple transient coexpression of the rice proteins in Nicotiana benthamiana. Secondary structure models of rice (plant) UreD and UreF proteins revealed a possible functional conservation to bacterial orthologs, especially for UreF. Using amino-terminally StrepII-tagged urease accessory proteins, an interaction between rice UreD and urease could be shown. Prokaryotic and eukaryotic urease activation complexes seem conserved despite limited protein sequence conservation for UreF and UreD. In plant metabolism, urea is generated by the arginase reaction. Rice arginase was transiently expressed as a carboxyl-terminally StrepII-tagged fusion protein in N. benthamiana, purified, and biochemically characterized (K(m) = 67 mm, k(cat) = 490 s(-1...Continue Reading

References

Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·I S ParkR P Hausinger
Jan 8, 1999·Plant Physiology·A Goldraij, J C Polacco
Mar 9, 1999·Plant Physiology·H J KronzuckerG J Kirk
Jan 29, 2000·The Plant Journal : for Cell and Molecular Biology·S K FreyermuthJ C Polacco
Apr 6, 2001·Plant Molecular Biology·C P WitteM A Taylor
Dec 10, 2002·Molecular Genetics and Genomics : MGG·M BacanamwoJ C Polacco
Aug 24, 2004·The Journal of Biological Chemistry·Hui ChenGregg A Howe
Dec 18, 2004·Plant Molecular Biology·Claus-Peter WitteTina Romeis
Jul 1, 1990·Plant Physiology·J H Kang, Y D Cho
Jul 28, 2006·Molecular Biology and Evolution·James J ElserSudhir Kumar
Nov 8, 2006·Nucleic Acids Research·Kevin L ChildsAgnes P Chan
Aug 4, 2007·The Plant Journal : for Cell and Molecular Biology·Soichi KojimaNicolaus von Wirén
May 30, 2008·Plant Physiology·Patricia MérigoutFrançoise Daniel-Vedele
Nov 26, 2009·Nature Chemical Biology·Andrea K WernerClaus-Peter Witte
Jan 5, 2010·Metallomics : Integrated Biometal Science·Eric L CarterRobert P Hausinger

❮ Previous
Next ❯

Citations

Mar 25, 2011·Journal of Experimental Botany·Joe C PolaccoKristin D Bilyeu
Mar 22, 2012·Plant & Cell Physiology·Antoine GravotMaria J Manzanares-Dauleux
Dec 26, 2012·Plant Science : an International Journal of Experimental Plant Biology·Joe C PolaccoTiago Tezotto
Jan 1, 2013·The Plant Journal : for Cell and Molecular Biology·Xuefeng MaJianmin Wan
Dec 3, 2014·The Plant Journal : for Cell and Molecular Biology·Anne BohnerNicolaus von Wirén
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Claus-Peter Witte
May 10, 2015·Plant & Cell Physiology·Huayiu YangUwe Ludewig
Mar 4, 2017·Genetics and Molecular Biology·Ciliana RechenmacherMaria H Bodanese-Zanettini
Mar 13, 2018·Environmental Microbiology·Serina L RobinsonLawrence P Wackett
Sep 16, 2017·Scientific Reports·Ayufu YilamujiangAxel Mithöfer
Feb 8, 2016·Molecular Biology Reports·Ertugrul FilizIbrahim Ilker Ozyigit
Apr 11, 2020·Frontiers in Plant Science·Santiago AlejandroEdgar Peiter
Dec 7, 2020·Physiologia Plantarum·Marcel P Beier, Soichi Kojima

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.