Identification and characterization of RAPD markers inferring genetic relationships among Pine species

Genome Génome / Conseil National De Recherches Canada
K K NkongoloW S Gratton

Abstract

Total genomic DNAs were extracted from several populations of pine species and amplified using oligonucleotides of random sequences. Polymorphism in random amplified polymorphic DNA (RAPD) markers was high and sufficient in distinguishing each of the species. Genetic relationships among eight pine species (Pinus sylvestris, Pinus strobus, Pinus rigida, Pinus resinosa, Pinus nigra, Pinus contorta, Pinus monticola, and Pinus banksiana) from different provenances were analyzed. The degree of band sharing was used to evaluate genetic distance between species and to construct a phylogenetic tree. In general, the dendrogram corroborated the description of relationships based on morphological characteristics and crossability, but also provided new insights into pine taxonomy. RAPD markers specific to some pine species were cloned and sequenced. PCR amplifications using pairs of designed specific primers revealed that all the cloned sequences were likely genus specific because they were not found in spruce or larch. True species-specific sequences were identified using designed primers flanking cloned RAPD fragments. The analysis of RAPD fragment sequences confirmed the genetic relationships among species. A 2281-bp RAPD band called PI...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R S ReiterP A Scolnik
Feb 1, 1992·Genome Génome / Conseil National De Recherches Canada·C S EchtT J McCoy
Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Apr 29, 1998·Genome Génome / Conseil National De Recherches Canada·S SvitashevR R Wang
Aug 1, 1996·Genome Génome / Conseil National De Recherches Canada·E F Marillia, G J Scoles
Apr 1, 1994·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·C HalldénT Säll
Jun 1, 1994·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·I AllonaC Aragoncillo
Mar 1, 1994·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J B Dos SantosM K Slocum
Feb 1, 1993·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·N A TinkerD E Mather
Aug 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·K M Devos, M D Gale
Dec 1, 1991·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M E DeveyD B Neale
Apr 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J WildeW Powell
Jul 1, 1990·Evolution; International Journal of Organic Evolution·Steven H Strauss, Allan H Doerksen

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Citations

Mar 1, 2012·Environmental Science & Technology·Eric A DubinskyGary L Andersen
Jul 2, 2009·Nature Reviews. Drug Discovery·Silvia Lapenna, Antonio Giordano
Dec 22, 2009·Molecular BioSystems·Pierre GeurtsLouis Wehenkel
Oct 22, 2010·Genome Génome / Conseil National De Recherches Canada·Melanie Mehes-SmithKabwe Nkongolo
Dec 4, 2012·Genome Génome / Conseil National De Recherches Canada·K K Nkongolo, M Mehes-Smith
Apr 2, 2014·TheScientificWorldJournal·Jianzhong WangJun Kong
Sep 28, 2004·Evolution; International Journal of Organic Evolution·Eva GrotkoppThomas L Rost
Feb 24, 2009·Journal of Zhejiang University. Science. B·Manuel Toral IbañezRafael M Navarro-Cerrillo
Feb 7, 2017·Chemistry & Biodiversity·Zorica S MitićPetar D Marin
Mar 1, 2011·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Parvathi Chary

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