PMID: 9431462Feb 12, 1998Paper

Identification and characterization of rat 364-kDa Golgi-associated protein recognized by autoantibodies from a patient with rheumatoid arthritis

Cell Structure and Function
C TokiY Ikehara

Abstract

Autoantibodies from a patient with rheumatoid arthritis recognized an antigen localized in the Golgi complex of various cells tested. The autoantibodies were used as a probe for screening rat NRK cDNA library, resulting in identification of an 11 kbp cDNA. The cDNA contained an open reading frame which encodes a 3,187-residue protein with a calculated mass of 364 kDa. The predicted protein, GCP364 (for a Golgi complex-associated protein of 364 kDa), was found to have no NH2-terminal signal sequence but a single hydrophobic domain at the COOH terminus and characteristically contain many coiled-coil domains with various sizes throughout the entire sequence. The identity of GCP364 with the autoantigen was confirmed by immunofluorescence and immunoblot analysis with the autoantibodies and anti-recombinant GCP364 produced in rabbits and by transfection/expression experiments. Search for the protein sequence data base revealed that GCP364 has 75% identity in amino acid sequence with human GCP372/giantin, indicating that it is a rat homolog of the latter. Immunogold electron microscopy showed that GCP364 was not detected on coated vesicles derived from the Golgi membrane, suggesting no involvement in the formation of transport vesicle...Continue Reading

Citations

Sep 9, 2004·The Journal of Experimental Medicine·Burkhard LudewigUgur Sahin
Apr 16, 2011·Cell and Tissue Research·Yi Xiang, Yanzhuang Wang
Feb 19, 2003·Biochemical and Biophysical Research Communications·Toshiyuki FujiwaraYukio Ikehara
Aug 17, 1999·Current Opinion in Cell Biology·D Shields, P Arvan
Jan 27, 1999·Biochemical and Biophysical Research Communications·T FujiwaraY Ikehara
Mar 4, 2010·Journal of Pharmacological Sciences·Nobuhiro Nakamura
Mar 30, 2004·Journal of Biochemistry·Shin-ichiro YoshimuraNobuhiro Nakamura
Dec 24, 1998·Lupus·C A von MühlenM J Fritzler
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Apr 10, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Pravin B Sehgal, Somshuvra Mukhopadhyay
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Sep 24, 2019·Frontiers in Cell and Developmental Biology·Ayano SatohKunihiko Nishino
Oct 3, 2003·The Journal of Biological Chemistry·Eiji OhtaYukio Ikehara

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