Identification and characterization of receptors for granulocyte colony-stimulating factor on human placenta and trophoblastic cells.

Proceedings of the National Academy of Sciences of the United States of America
H UzumakiY Umezawa

Abstract

Since radioiodination of human granulocyte colony-stimulating factor (G-CSF) is difficult, we synthesized a mutein of human G-CSF that retains full biological activity and receptor-binding capacity for at least 2 weeks after radioiodination. Receptors for human G-CSF were characterized in the plasma membrane fraction from the human term placenta (human placental membranes) and trophoblastic cells by using the 125I-labeled mutein of human G-CSF (KW-2228). The specific binding of 125I-labeled KW-2228 to placental membranes was pH-dependent, with maximal specific binding at pH 7.8; it increased linearly with protein to 3.7 mg of protein per ml and was both time- and temperature-dependent, with maximal binding at 4 degrees C after a 24-hr incubation. When we examined the ability of hematopoietic growth factors to inhibit 125I-labeled KW-2228 binding, we found that KW-2228 and intact human G-CSF inhibited 125I-labeled KW-2228 binding, whereas erythropoietin or granulocyte-macrophage colony-stimulating factor did not. Scatchard analysis revealed a single receptor type with a Bmax of 210 fmol/mg of protein and a Kd of 480 pM. The human G-CSF receptors on human placental membranes were shown to consist of two molecular species of 150 k...Continue Reading

Citations

Jul 1, 1991·International Journal of Cell Cloning·M F BrizziL Pegoraro
Sep 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J YooC W Beattie
Jan 1, 1995·Archives of Gynecology and Obstetrics·E WeimannM J Lentze
Aug 9, 2005·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Kazuya EndoYoshihiko Maehara
Feb 1, 1990·Research in Immunology·T Wegmann
Oct 1, 1991·Current Opinion in Immunology·J W Pollard
Apr 6, 2004·Developmental and Comparative Immunology·Daniel R BarredaMiodrag Belosevic
Mar 10, 2001·Journal of Dermatological Science·K HiraiM Fukuda
Mar 10, 2001·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·E. B. NinciT. Bauknecht
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R FukunagaS Nagata
Nov 1, 1995·European Journal of Clinical Investigation·S MakinodaS Fujimoto
Feb 1, 1994·Journal of Medical Primatology·T G GolosK T Schultz
Jan 1, 1995·Infectious Diseases in Obstetrics and Gynecology·B D RaynorP Duff
Jun 1, 1992·Journal of Clinical Pharmacology·J M Rowe, A P Rapoport
Apr 1, 1993·The American Journal of the Medical Sciences·R A Fleischman
Jul 1, 1992·Baillière's Clinical Haematology·O KanA D Whetton
Feb 26, 2016·Gene·Janette Mareska RumbajanKeiichiro Joh
Sep 11, 2010·The American Journal of Pathology·S Joseph HuangCharles J Lockwood
Sep 1, 1992·Baillière's Clinical Obstetrics and Gynaecology·J N Bulmer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.