Identification and characterization of single-nucleotide polymorphisms in MCH-R1 and MCH-R2

Obesity Research
B E HawesJason S Simon

Abstract

To identify and functionally characterize single-nucleotide polymorphisms (SNPs) in melanin-concentrating hormone (MCH)-R1 and -R2. The entire coding regions and intron/exon splice junction regions of MCH-R1 and MCH-R2 were sequenced from anonymous white (n=45) and African-American (n=46) individuals. DNA was analyzed, and SNPs were identified using Phred, Phrap, and Consed software. DNA constructs containing MCH-R1 and MCH-R2 SNPs were generated and expressed in CHO cells. The effect of the SNPs in MCH-R1 and MCH-R2 were assessed in receptor binding assays and functional assays measuring changes in intracellular cAMP and Ca2+ levels. We identified 12 SNPs in the MCH-R1 gene. Two of these SNPs are in coding regions, and one produces an arginine-for-glycine substitution at residue 34 in the MCH-R1 sequence. This SNP is present at a minor allele frequency of 15% in the African-American population tested in this study. We identified eight SNPs in the MCH-R2 gene. Four of these SNPs are in coding regions, and two produce amino acid substitutions. Lysine substitutes for arginine at residue 63 of the African-American population, and glutamine substitutes for arginine at residue 152 in whites (minor allele frequency of 2% for both SNP...Continue Reading

References

Jan 1, 1999·Nature·M ShimadaE Maratos-Flier
Aug 12, 1999·Biochemical and Biophysical Research Communications·Y ShimomuraM Fujino
Feb 13, 2001·The Journal of Clinical Investigation·D S LudwigE Maratos-Flier
Mar 29, 2001·The Journal of Biological Chemistry·J HillA I Muir
May 18, 2001·Biochemical and Biophysical Research Communications·M MoriM Fujino
Jun 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·A W SailerQ Liu
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·S AnK Dai
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Donald J MarshSu Qian
Oct 2, 2002·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·O Della-ZuanaN Levens
Nov 28, 2002·American Journal of Physiology. Endocrinology and Metabolism·Akira GomoriAkio Kanatani

❮ Previous
Next ❯

Citations

Mar 10, 2006·The International Journal of Biochemistry & Cell Biology·Dennis FryChristine A Collins
Mar 22, 2006·Expert Opinion on Therapeutic Targets·Guillaume J Hervieu
Nov 19, 2010·Journal of Receptor and Signal Transduction Research·Alex N EberleUrs Zumsteg
Aug 19, 2007·The Journal of Clinical Endocrinology and Metabolism·Maya GhoussainiDavid Meyre
Nov 28, 2006·Journal of Medicinal Chemistry·Francis X TavaresHui-Qiang Zhou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.