Identification and characterization of SNJ2, the first temperate pleolipovirus integrating into the genome of the SNJ1-lysogenic archaeal strain

Molecular Microbiology
Ying LiuXiangdong Chen

Abstract

Proviral regions have been identified in the genomes of many haloarchaea, but only a few archaeal halophilic temperate viruses have been studied. Here, we report a new virus, SNJ2, originating from archaeal strain Natrinema sp. J7-1. We demonstrate that this temperate virus coexists with SNJ1 virus and is dependent on SNJ1 for efficient production. Here, we show that SNJ1 is an icosahedral membrane-containing virus, whereas SNJ2 is a pleomorphic one. Instead of producing progeny virions and forming plaques, SNJ2 integrates into the host tRNA(Met) gene. The virion contains a discontinuous, circular, double-stranded DNA genome of 16 992 bp, in which both nicks and single-stranded regions are present preceded by a 'GCCCA' motif. Among 25 putative SNJ2 open reading frames (ORFs), five of them form a cluster of conserved ORFs homologous to archaeal pleolipoviruses isolated from hypersaline environments. Two structural protein encoding genes in the conserved cluster were verified in SNJ2. Furthermore, SNJ2-like proviruses containing the conserved gene cluster were identified in the chromosomes of archaea belonging to 10 different genera. Comparison of SNJ2 and these proviruses suggests that they employ a similar integration strategy ...Continue Reading

References

Jan 1, 1979·Journal of Virology·A R ShawD J McCorquodale
Apr 19, 1974·Nature·T Torsvik, I D Dundas
Sep 15, 1997·Nucleic Acids Research·D Esposito, J J Scocca
Mar 21, 1998·Nucleic Acids Research·A V Lukashin, M Borodovsky
Mar 22, 2000·Biochemistry·A CorcelliM Kates
Aug 9, 2002·Theoretical Population Biology·Dennis H BamfordDavid I Stuart
Sep 18, 2002·Research in Microbiology·Qunxin SheLanming Chen
Jun 12, 2003·Research in Microbiology·Dennis H Bamford
Jun 12, 2003·Research in Microbiology·Allan Campbell
Mar 30, 2004·Biochemical Society Transactions·Q SheL Chen
Jan 22, 2005·Virology·Jianbin WangSongnian Hu
May 7, 2005·Archaea : an International Microbiological Journal·Ren Zhang, Chun-Ting Zhang
Feb 6, 2007·Trends in Microbiology·Caroline E LilleyMatthew D Weitzman
Apr 5, 2007·Nature Protocols·Hermann Schägger
Nov 21, 2007·Canadian Journal of Microbiology·Yunjun MeiXiangdong Chen
Mar 21, 2009·Molecular Microbiology·Maija K PietiläDennis H Bamford
Jan 22, 2010·Journal of Virology·Elina RoineDennis H Bamford
Aug 10, 2010·Annual Review of Microbiology·Matthew D WeitzmanMira S Chaurushiya
May 17, 2011·FEMS Microbiology Reviews·Mery PinaDavid Prangishvili
Jun 28, 2011·PloS One·Mike L Dyall-SmithDieter Oesterhelt
Feb 24, 2012·Journal of Virology·Maija K PietiläDennis H Bamford
Mar 1, 2012·Cell Cycle·Sarah SerticMarco Muzi-Falconi
Mar 8, 2012·Nucleic Acids Research·Ana SenciloElina Roine
Nov 21, 2012·Environmental Microbiology·Maija K PietiläDennis H Bamford
Jan 30, 2013·Biochemical Society Transactions·Jörg Soppa
Apr 16, 2013·Archaea : an International Microbiological Journal·Kate PorterMike Dyall-Smith

❮ Previous
Next ❯

Citations

Sep 30, 2015·Molecular Microbiology·Mike Dyall-Smith, Kate Porter
Mar 2, 2016·Environmental Microbiology Reports·Nina S AtanasovaHanna M Oksanen
Jan 24, 2018·Nucleic Acids Research·Jiao WangXiangdong Chen
Mar 2, 2016·Viruses·Julija SvirskaitėDennis H Bamford
Mar 3, 2018·Genes·Jacob H Munson-McGeeMark J Young
Apr 21, 2017·Journal of Virology·Ying LiuDavid Prangishvili
Mar 29, 2019·Environmental Microbiology·Carolina M MizunoHanna M Oksanen
Jun 8, 2018·Frontiers in Microbiology·Mike Dyall-Smith, Friedhelm Pfeiffer
Jun 26, 2020·Archives of Virology·Tatiana A Demina, Hanna M Oksanen
Jul 16, 2020·Nature Reviews. Microbiology·Mart KrupovicEugene V Koonin
Mar 30, 2017·Archaea : an International Microbiological Journal·Yuchen WangXiangdong Chen
May 16, 2017·Nature Reviews. Microbiology·Alexander WagnerSonja-Verena Albers
Feb 2, 2021·FEMS Microbiology Reviews·Catherine BadelJacques Oberto

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.