PMID: 12379462Oct 16, 2002Paper

Identification and characterization of SnrA, an inducible oxygen-insensitive nitroreductase in Salmonella enterica serovar Typhimurium TA1535

Mutation Research
M R NokhbehI B Lambert

Abstract

The biological activity of many nitrosubstituted compounds, many of which are produced commercially or have been identified as environmental contaminants, is dependent on metabolic activation catalyzed by nitroreductases. In the current study, we have cloned a nitroreductase gene, Salmonella typhimurium nitroreductase A (snrA), from S. enterica serovar Typhimurium strain TA1535, and characterized the purified gene product. SnrA is 240 amino acids in length and shares 87% sequence identity to the Escherichia coli homolog, E. coli nitroreductase A (NfsA). SnrA is the major nitroreductase in S. enterica serovar Typhimurium strain TA1535 and catalyzes nitroreduction through a ping-pong bi-bi mechanism in a NADPH and flavine mononucleotide (FMN) dependent manner. SnrA exhibits extremely low levels of FMN reductase activity but the nitroreductase activity of SnrA is competitively inhibited by exogenously added FMN. Treatment of TA1535 with paraquat resulted in induction of nitroreductase activity, suggesting that SnrA is a member of the S. enterica serovar Typhimurium SoxRS regulon associated with cellular defense against oxidative damage. Examination of the microbial genomes databases shows that SnrA homologs are widely distributed ...Continue Reading

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Citations

May 31, 2007·Applied Microbiology and Biotechnology·Barth F SmetsAbraham Esteve-Nuñez
Dec 8, 2005·Applied and Environmental Microbiology·Eva Pérez-ReinadoM Dolores Roldán
Mar 22, 2008·Environmental Microbiology·Eva Pérez-ReinadoConrado Moreno-Vivián
Jul 14, 2005·Environmental Microbiology·Antonio CaballeroAbraham Esteve-Núñez
Jul 12, 2008·Environmental and Molecular Mutagenesis·Jóhannes ReynissonVolker M Arlt
Mar 22, 2008·FEMS Microbiology Reviews·María Dolores RoldánConrado Moreno-Vivián
Dec 19, 2016·Regulatory Toxicology and Pharmacology : RTP·Erina HanakiMakoto Matsumoto
May 8, 2010·Organic & Biomolecular Chemistry·Yanto YantoAndreas S Bommarius
Oct 7, 2021·Applied and Environmental Microbiology·Yao ZhouShengmin Zhou
May 2, 2012·Bioorganic & Medicinal Chemistry·Ayhan Çelik, Gülden Yetiş
Jun 13, 2012·Biochemical and Biophysical Research Communications·Seo Young BangSung Goo Park

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