Identification and characterization of Sr13 , a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group

Proceedings of the National Academy of Sciences of the United States of America
Wenjun ZhangJorge Dubcovsky

Abstract

The Puccinia graminis f. sp. tritici (Pgt) Ug99 race group is virulent to most stem rust resistance genes currently deployed in wheat and poses a threat to global wheat production. The durum wheat (Triticum turgidum ssp. durum) gene Sr13 confers resistance to Ug99 and other virulent races, and is more effective at high temperatures. Using map-based cloning, we delimited a candidate region including two linked genes encoding coiled-coil nucleotide-binding leucine-rich repeat proteins designated CNL3 and CNL13. Three independent truncation mutations identified in each of these genes demonstrated that only CNL13 was required for Ug99 resistance. Transformation of an 8-kb genomic sequence including CNL13 into the susceptible wheat variety Fielder was sufficient to confer resistance to Ug99, confirming that CNL13 is Sr13CNL13 transcripts were slightly down-regulated 2-6 days after Pgt inoculation and were not affected by temperature. By contrast, six pathogenesis-related (PR) genes were up-regulated at high temperatures only when both Sr13 and Pgt were present, suggesting that they may contribute to the high temperature resistance mechanism. We identified three Sr13-resistant haplotypes, which were present in one-third of cultivated...Continue Reading

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Citations

May 17, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Junli ZhangJorge Dubcovsky
Jul 7, 2019·G3 : Genes - Genomes - Genetics·Meriem AounMaricelis Acevedo
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May 31, 2021·Current Opinion in Plant Biology·Javier Sánchez-Martín, Beat Keller
Nov 2, 2021·Frontiers in Plant Science·Hongna LiShisheng Chen

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Datasets Mentioned

BETA
EX24785

Methods Mentioned

BETA
Transgenic
Assay
PCR
fluorescence microscopy
PCRs

Software Mentioned

MEGA
BLASTN
SAS
Galaxy
BLASTX
Sequin
Phyre
ASSES

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