PMID: 9421507Feb 28, 1998Paper

Identification and characterization of srp1, a gene of fission yeast encoding a RNA binding domain and a RS domain typical of SR splicing factors

Nucleic Acids Research
T GrossN F Käufer

Abstract

The SR protein family is involved in constitutive and regulated pre-mRNA splicing and has been found to be evolutionarily conserved in metazoan organisms. In contrast, the genome of the unicellular yeast Saccharomyces cerevisiae does not contain genes encoding typical SR proteins. The mammalian SR proteins consist of one or two characteristic RNA binding domains (RBD), containing the signature sequences RDAEDA and SWQDLKD respectively, and a RS (arginine/serine-rich) domain which gave the family its name. We have now cloned from the fission yeast Schizosaccharomyces pombe the gene srp1. This gene is the first yeast gene encoding a protein with typical features of mammalian SR protein family members. The gene is not essential for growth. We show that overexpression of the RNA binding domain inhibits pre-mRNA splicing and that the highly conserved sequence RDAEDA in the RBD is involved. Overexpression of Srp1 containing mutations in the RS domain also inhibits pre-mRNA splicing activity. Furthermore, we show that overexpression of Srp1 and overexpression of the mammalian SR splicing factor ASF/SF2 suppress the pre-mRNA splicing defect of the temperature-sensitive prp4-73 allele. prp4 encodes a protein kinase involved in pre-mRNA ...Continue Reading

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Citations

Feb 13, 2003·Nucleic Acids Research·Elisabeth BonClaude Gaillardin
Nov 10, 2005·Eukaryotic Cell·Caroline C L JenkinsChris J Norbury
Oct 12, 2000·Molecular and Cellular Biology·C M RomfoJ A Wise
May 4, 2004·Molecular and Cellular Biology·Christopher J Webb, Jo Ann Wise
Oct 28, 2009·Genome Biology·Peter J Shepard, Klemens J Hertel
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·C W SiebelX D Fu
Jun 15, 1999·The Journal of Cell Biology·N KataokaG Dreyfuss
Jun 12, 2012·Experimental Cell Research·Zhaohua TangAntony Carr
Jul 13, 2011·Experimental Cell Research·Zhaohua TangAntony Carr
Jul 29, 2011·The FEBS Journal·Laure TwyffelsVéronique Kruys
Aug 16, 2005·Fungal Genetics and Biology : FG & B·Brian D Shaw, Srijana Upadhyay
Apr 20, 2011·Journal of Molecular Biology·Nitish MittalSarath Chandra Janga

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