Identification and characterization of suppressor of cytokine signaling 3 (SOCS-3) homologues in teleost fish

Molecular Immunology
Hong-Jian JinLi-Xin Xiang

Abstract

The suppressor of cytokine signaling 3 (SOCS-3) is a member of a newly discovered protein family, which have been shown to regulate the responses of many immune cytokines, such as interferon (IFN), interleukin-2 (IL-2) and IL-6, etc., by inhibiting Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in a negative auto-regulatory manner. Although SOCS-3 was well characterized in several mammal species, there was still no report in fish. In present study, we initially identified and characterized the SOCS-3 genes from three fishes, the Tetraodon nigroviridis, the Danio rerio and the Fugu rubripes. The results showed that Tetraodon SOCS-3 gene located within a 2666 bp genomic fragment of chromosome 3, transcribed into a 1445 bp mRNA including 273 bp 5' UTR (untranslated region), 606 bp ORF (open reading frame) and 566 bp 3' UTR. Tetraodon SOCS-3 with 201aa (amino acid) has a calculated molecular mass of 22.76 kDa and a theoretical pI of 8.99. Danio SOCS-3 gene located within a 3617 bp genomic fragment of chromosome 3, transcribed into a 1927 bp mRNA including 178 bp 5' UTR, 624 bp ORF and 1125 bp 3' UTR. Danio SOCS-3 with 207aa has a calculated molecular mass of 23.68 kDa and a theoretical pI of ...Continue Reading

Citations

Jan 10, 2008·Blood·Duncan Carradice, Graham J Lieschke
Dec 29, 2011·Journal of Signal Transduction·Tiehui WangChristopher J Secombes
Aug 11, 2012·Developmental and Comparative Immunology·Anju M PhilipMathilakath M Vijayan
Apr 17, 2012·Developmental and Comparative Immunology·Lv-yun ZhuJian-zhong Shao
Jul 26, 2011·Developmental and Comparative Immunology·Jun Zou, Christopher J Secombes
Oct 29, 2008·General and Comparative Endocrinology·Ana Lupe Motta StudzinskiLuis Fernando Marins
Jun 20, 2008·Fish & Shellfish Immunology·Chris Secombes
Apr 20, 2007·Developmental and Comparative Immunology·Hong-Jian JinJian-Zhong Shao

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