Identification and characterization of syntaxin 1 antisense variants in Limulus polyphemus.

Cellular and Molecular Neurobiology
Z CaoJ G Townsel

Abstract

1.A Limulus SMART(TM) cDNA library screening resulted in the cloning of four syntaxin 1 homologs (referred to as Limulus syntaxin [Lim-syn] 1A, 1B, 1C, and 1D) (Wang, Y., Cao, Z., Xu, W., Kemp, M. D., McAdory, B. S., Newkirk, R. F., Ivy, M. T., and Townsel, J. G. (2004). Gene 326:189-199) and two novel intron-retaining syntaxin 1-like variants, designated Limulus syntaxin variant [Lim-synV] 1A/1C and Lim-synV 1B/1D. 2.The variants exhibited high amino acid sequence identity with the four syntaxin 1 homologs. Specifically, Lim-synV 1A/1C and Lim-synV 1B/1D were homologous to Lim-syn 1A/1C and Lim-syn 1B/1D, respectively. Surprisingly, both Lim-synV 1A/1C and 1B/1D are unusual in that each has a poly A+ tail, an intron, and the common splice motif "GT-AG" at the intron-exon boundary. Exons one and two on the complementary transcript of Lim-synV 1B/1D are separated by a 150 bp intron beginning at #95/96 of the predicted sequences for Lim-syn 1B and 1D, respectively. 3. In contrast, examination of the approximately 3.17 kb Lim-synV 1A/1C clone indicated the inclusion of an insert of 1120 base pairs (bp) beginning at codon #37/38 of the predicted Lim-syn 1A and 1C cDNAs' open reading frames (ORFs). Further, the intron sequence of Li...Continue Reading

References

Jun 26, 1995·Biochemical and Biophysical Research Communications·K IbarakiT Abe
Sep 10, 1993·Cell·M K BennettR H Scheller
Mar 1, 1996·The Journal of Biological Chemistry·M A TehraniC Kamibayashi
Jan 1, 1997·The Biochemical Journal·M N JagadishC W Ward
May 20, 1998·European Journal of Cell Biology·A SimonsenH Stenmark
Jun 24, 1999·Cellular and Molecular Life Sciences : CMLS·J E Gerst
Mar 31, 2000·Pharmacology & Therapeutics·D Mercatante, R Kole
Jul 20, 2000·Cellular and Molecular Neurobiology·Y WangJ G Townsel
Sep 23, 2000·Nature·F ParlatiJ E Rothman
Dec 12, 2001·Genome Biology·F Y TengB L Tang
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Francesco ParlatiJames E Rothman
Sep 3, 2004·The Plant Journal : for Cell and Molecular Biology·Hadas Ner-GaonRobert Fluhr

❮ Previous
Next ❯

Citations

Oct 10, 2006·BMC Genomics·Sébastien BesteiroJeremy C Mottram
Apr 9, 2008·Hemodialysis International·Rajiv Sharma, Mitchell H Rosner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.