Identification and Characterization of T5-Like Bacteriophages Representing Two Novel Subgroups from Food Products

Frontiers in Microbiology
Domonkos SvábIstván Tóth

Abstract

During recent years, interest in the use of bacteriophages as biocontrol agents against foodborne pathogens has increased, particularly for members of the family Enterobacteriaceae, with pathogenicEscherichia coli, Shigella, and Salmonellastrains among them. Here, we report the isolation and characterisation of 12 novel T5-like bacteriophages from confiscated food samples. All bacterophages effectively lysedE. coliK-12 strains and were able to infect pathogenicE. colistrains representing enterohaemorrhagic (EHEC), enteropathogenic (EPEC), enterotoxigenic (ETEC), and enteroinvasive (EIEC) pathotypes,Shigella dysenteriae, S. sonneistrains, as well as multidrug-resistant (MDR)E. coliand multiple strains representing differentSalmonella entericaserovars. All the bacteriophages exhibitedSiphoviridaemorphology. Whole genome sequencing of the novel T5-like bacteriophages showed that they represent two distinct groups, with the genome-based grouping correlating to the different host spectra. As these bacteriophages are of food origin, their stability and lack of any virulence genes, as well as their broad and mutually complementary host spectrum makes these new T5-like bacteriophages valuable candidates for use as biocontrol agents aga...Continue Reading

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Citations

Dec 7, 2018·Archives of Virology·Alla K GolomidovaAndrey V Letarov
Mar 19, 2019·Environmental Microbiology·Slawomir MichniewskiAndrew D Millard
Nov 19, 2019·Frontiers in Microbiology·Cátia PacíficoFriederike Hilbert
Jan 12, 2021·Biosensors & Bioelectronics·Wajid HussainShenqi Wang
Jul 14, 2021·Applied Microbiology and Biotechnology·Edgar González-VillalobosJosé Molina-López

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Datasets Mentioned

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AY543070.1

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transmission electron microscopy

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