Identification and characterization of the factor H and FHL-1 binding complement regulator-acquiring surface protein 1 of the Lyme disease spirochete Borrelia spielmanii sp. nov

International Journal of Medical Microbiology : IJMM
Pia HerzbergerPeter Kraiczy

Abstract

Borrelia spielmanii, one of the etiological agents of Lyme disease found in Europe, evades host complement-mediated killing by recruitment of the immune regulators factor H and FHL-1 from human serum. Serum-resistant and intermediate serum-resistant isolates express up to 3 distinct complement regulator-acquiring surface proteins (CRASPs) that bind factor H and/or FHL-1. The present study describes identification and functional characterization of BsCRASP-1 as the dominant factor H and FHL-1 binding protein of B. spielmanii. BsCRASP-1 is a 27.7kDa outer surface lipoprotein, which after processing has a predicted mass of 24.9kDa. BsCRASP-1 is encoded by a single copy gene, cspA, that maps to a linear plasmid of approximately 55kb. Ligand affinity blot techniques revealed that both native and recombinant BsCRASP-1 from different isolates can strongly bind FHL-1, but only weakly factor H. Deletion mutants of recombinant BsCRASP-1 were generated and a high-affinity binding site for factor H and FHL-1 was mapped to its carboxy-terminal 10-amino-acid residue domain. Similarly, the dominant binding site of factor H and FHL-1 was localized to short consensus repeats (SCRs) 5-7. Factor H and FHL-1 maintained cofactor activity for factor...Continue Reading

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Citations

Apr 14, 2010·Survey of Ophthalmology·Larry A DonosoHelena Kuivaniemi
Apr 27, 2013·Biochemical and Biophysical Research Communications·Kalvis BrangulisKaspars Tars
Oct 12, 2016·Frontiers in Immunology·Peter Kraiczy
Nov 6, 2013·Infection and Immunity·Claudia HammerschmidtPeter Kraiczy
Feb 13, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arthur DoplerChristoph Q Schmidt
Jul 19, 2013·The Journal of Biological Chemistry·Arno KoenigsPeter Kraiczy
Mar 16, 2021·Frontiers in Immunology·Sara R MooreViviana P Ferreira

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