Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans

PloS One
Cláudia S L VicentePaulo Vieira

Abstract

Similar to other plant-parasitic nematodes, root lesion nematodes possess an array of enzymes that are involved in the degradation of the plant cell wall. Here we report the identification of a gene encoding a cell wall-degrading enzyme, pectin methylesterase PME (EC 3.1.1.11), in the root lesion nematode Pratylenchus penetrans. Both genomic and coding sequences of the gene were cloned for this species, that included the presence of four introns which eliminated a possible contamination from bacteria. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. Temporal expression of Pp-pme in planta was validated at early time points of infection. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato virus X-based vector. To our knowledge, this is the first report on identification and characterization of a PME gene within the phylum Nematoda.

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Datasets Mentioned

BETA
PXD012419
MK295632

Methods Mentioned

BETA
PCR
deamination

Software Mentioned

CLC Main Workbench
BLASTp
MUSCLE
SignalP
AMINOSAN
PRIDE Inspector
Mascot
PeptideShaker
TRIMAL
ProteParam

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