Identification and characterization of the genes encoding three structural proteins of the Thermoproteus tenax virus TTV1

Molecular & General Genetics : MGG
H NeumannW Zillig

Abstract

Three structural proteins, TP1, TP2 and TP3, of the virus TTV1 of the thermophilic archaebacterium Thermoproteus tenax strain Kra1 were mapped within the viral genome by locating the amino-terminal amino acid sequences of these proteins in the TTV1 DNA sequence. The derived amino acid sequences comprise 113, 139 and 160 amino acid residues, respectively. All three proteins are hydrophobic. The three genes are not linked, but transcribed in the same direction. No Shine-Dalgarno sequences are found in the vicinity of the initiation codons of these three genes. By Northern analysis, four mRNAs of 0.5 kb, 0.8 kb, 1.1 kb and 1.8 kb in size were found to be encoded in the region and in the vicinity of the genes, the shortest one (t1) encoding TP3 and the longest one (t4) encoding TP2. No transcript from the region encoding TP1 has been found so far. A transcriptional start site was mapped for the transcript t1. Its upstream sequence was similar to the putative consensus sequence for archaebacterial promoters.

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Citations

Jul 1, 1990·Molecular & General Genetics : MGG·H Neumann, W Zillig
Sep 23, 2003·Research in Microbiology·Jamie C SnyderMark J Young
Oct 17, 2006·Nature Reviews. Microbiology·David PrangishviliRoger A Garrett
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C SchleperW Zillig
Nov 25, 1989·Nucleic Acids Research·H Neumann, W Zillig
Jan 11, 1990·Nucleic Acids Research·H Neumann, W Zillig
Jun 11, 1992·Nucleic Acids Research·E D LampertiL Villa-Komaroff
Mar 11, 1992·Nucleic Acids Research·E V Koonin
Feb 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Elena Ilka RensenDavid Prangishvili
Mar 1, 2006·Virus Research·David PrangishviliEugene V Koonin
Mar 6, 2007·Virology·Eric T LarsonC Martin Lawrence
Dec 13, 2018·The Journal of General Virology·David Prangishvili Ictv Report Consortium
Sep 13, 2005·Trends in Microbiology·David Prangishvili, Roger A Garrett

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