Identification and characterization of the guinea-pig cytomegalovirus glycoprotein H gene

Archives of Virology
R C Brady, Mark R Schleiss

Abstract

Subunit vaccines which target viral envelope glycoproteins offer promise for the prevention of congenital cytomegalovirus (CMV) infection. The guinea pig model of CMV infection is uniquely well suited to testing vaccines for prevention of congenital infection, since, in contrast to other animal cytomegaloviruses, the guinea pig CMV (GPCMV) crosses the placenta, producing intrauterine infection. Antibody to the CMV glycoproteins B (gB) and H (gH) appears to be important in conferring protective immunity. Unfortunately, little is known about specific GPCMV envelope glycoproteins. Sequencing of GPCMV genome fragments was therefore undertaken to test whether GPCMV encodes a gH homologue. Partial sequencing of the Hind III A fragment of the GPCMV genome revealed an open reading frame of 2,169 nucleotides capable of encoding a protein of 723 amino acids. Computer matrix analyses demonstrated identity between this ORF and the gH coding sequences of other herpesviruses. The GPCMV gH ORF encodes 12 highly conserved cysteine residues, contains 9 potential N-linked glycosylation sites, and has a predicted M(r) of 81.6 kDa. Northern blot hybridizations with gH-specific probes identified an abundant 5.1 kb mRNA with expression kinetics of a...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Mar 5, 1992·The New England Journal of Medicine·K B FowlerC A Alford
Feb 1, 1992·The Pediatric Infectious Disease Journal·S B BoppanaC A Alford
Jul 1, 1992·The Journal of General Virology·J XuA A Scalzo
Nov 1, 1991·The Journal of Infectious Diseases·L RasmussenT C Merigan
Feb 15, 1990·Analytical Biochemistry·M Stürzl, W K Roth
Jan 11, 1992·Nucleic Acids Research·S Faisst, S Meyer
Apr 1, 1990·Journal of Virology·C Y YinH C Isom
Jan 1, 1990·Current Topics in Microbiology and Immunology·M S CheeJ A Martignetti
Jan 1, 1990·Current Topics in Microbiology and Immunology·D H SpectorD A Wright
Sep 1, 1990·Reviews of Infectious Diseases·S A PlotkinK Brayman
Aug 1, 1985·Journal of Virology·B P GriffithE Goff
Dec 1, 1985·Journal of Medical Virology·M P LandiniM La Placa
Jan 1, 1987·Journal of Virology·T KouzaridesB G Barrell
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L E RasmussenT C Merigan
Jan 1, 1983·Methods in Enzymology·L Jennes, W E Stumpf
Oct 1, 1995·The Journal of Infectious Diseases·D F BratcherD I Bernstein
Jan 1, 1994·The Journal of General Virology·M RappU H Koszinowski

❮ Previous
Next ❯

Related Concepts

© 2022 Meta ULC. All rights reserved