Identification and characterization of the main beta-alanine uptake system in Escherichia coli

Applied Microbiology and Biotechnology
Frank SchneiderAndreas Burkovski

Abstract

In Escherichia coli, beta-alanine is a direct precursor in the biosynthesis of pantothenic acid (vitamin B5). Although a sufficient beta-alanine supply is crucial for biotechnological vitamin B5 production, nothing was known about beta-alanine transport in E. coli until now. The aim of this work was the characterization of beta-alanine transport by E. coli and the identification and overexpression of the corresponding carrier-encoding gene for the rational improvement of pantothenic acid-producing strains. beta-Alanine uptake was found to be an active process catalyzed by the amino acid carrier CycA. The corresponding gene was cloned and overexpressed, resulting in an increase in the uptake rate, compared with the wild type. In all tested strains, this overexpression led to a strong sensitivity to beta-alanine, but not to the other CycA substrates, such as L-alanine, D-alanine, and glycine. This prevented a direct application for the improvement of pantothenic acid-producing strains by an enhanced precursor supply.

Citations

May 3, 2011·Applied and Environmental Microbiology·Hatsuhiro HoriRyoichi Katsumata
Sep 6, 2006·Journal of Bacteriology·Andrew T Anfora, Rodney A Welch
Sep 1, 2009·Journal of Bacteriology·Olivia NeubauerThomas Eitinger
Jan 15, 2013·Journal of Bacteriology·Gary BaisaRodney A Welch
Jan 5, 2008·FEMS Microbiology Reviews·Christina SpryKevin J Saliba
Dec 21, 2014·BMC Biology·Daniel R G Price, Alex C C Wilson
Nov 27, 2018·Bioscience, Biotechnology, and Biochemistry·Yusuke YoshidaHiroya Yurimoto
Sep 28, 2007·Natural Product Reports·Michael E WebbAlison G Smith
Oct 9, 2019·International Journal of Molecular Sciences·Satoshi KatsubeHiroshi Yoneyama
Mar 15, 2020·Microbial Cell Factories·Chenyang WangZhijun Zhao
Nov 1, 2005·EcoSal Plus·Larry Reitzer
Apr 1, 2007·EcoSal Plus·Roberta Leonardi, Suzanne Jackowski
Apr 4, 2019·Metabolic Engineering·Volker F Wendisch

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.