Identification and characterization of the major cell envelope proteins of oral strains of Actinobacillus actinomycetemcomitans.

Infection and Immunity
J M Di Rienzo, E L Spieler

Abstract

The major cell envelope protein compositions of seven Actinobacillus actinomycetemcomitans strains of human origin were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The major envelope polypeptides were homogeneous, in relation to molecular weight, in all of the strains that were examined. The characterization of the five major proteins, designated Env1 through Env5, in the leukotoxic strain Y4 revealed that proteins Env2 to -5 may reside in the outer membrane as suggested by differential detergent extractions and 125I-labeling experiments. The proteins did not demonstrate covalent or ionic interactions with the peptidoglycan; however, one protein, Env2, displayed heat-modifiable properties, having apparent molecular weights of 32,000 and 45,000 when heated in sodium dodecyl sulfate at 50 and 100 degrees C, respectively. The protein composition of the extracellular "bleb" material, normally released by strain Y4, was determined, and proteins Env1 to -4 were the predominant protein species found. A comparison of the cell envelope proteins of strain Y4 with those of other members of the human oral flora, including species within the genera Capnocytophaga, Bacteroides, and Fusobacterium, revealed distinct ...Continue Reading

References

Mar 1, 1977·Journal of Periodontal Research·M G Newman, S S Socransky
Oct 1, 1979·Journal of Clinical Periodontology·A C TannerS S Socransky
Jan 1, 1978·Annual Review of Biochemistry·J M DiRienzoM Inouye
Jul 1, 1976·Journal of Periodontology·M G NewmanA Crawford
Aug 1, 1975·The Journal of Experimental Medicine·F MelchersC Galanos
Sep 1, 1967·Neurology·B F MartinJ Ransohoff
Sep 1, 1973·American Journal of Clinical Pathology·L W BurgherF Ware
Apr 1, 1980·Infection and Immunity·Y H ChenR I Mishell
Sep 1, 1964·Journal of Clinical Pathology·R G MITCHELL, W A GILLESPIE

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Citations

Jan 1, 1991·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·S C Holt, T E Bramanti
Apr 1, 1990·Oral Microbiology and Immunology·J M DiRienzoJ Slots
Feb 1, 1995·Oral Microbiology and Immunology·K R AlugupalliA S Naidu
Mar 1, 1989·Oral Microbiology and Immunology·D BeightonN W Johnson
Jan 1, 1990·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J L Ebersole
Apr 1, 1991·Oral Microbiology and Immunology·J M DiRienzoH Boehringer
May 1, 1996·Immunological Investigations·J L EbersoleM J Steffen
Sep 1, 1987·Oral Microbiology and Immunology·N S TaichmanJ Slots
Oct 1, 1984·Infection and Immunity·B Appelbaum, B Rosan
May 1, 1984·Infection and Immunity·J M DiRienzo, B Rosan
Jan 1, 1985·Infection and Immunity·J M DiRienzoE T Lally
Oct 1, 1990·Microbial Pathogenesis·D MullerE J Ruitenberg
Nov 1, 1994·Journal of Clinical Microbiology·T J van SteenbergenJ de Graaff

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