PMID: 8955385Dec 1, 1996Paper

Identification and characterization of the tolQRA genes of Pseudomonas aeruginosa

Journal of Bacteriology
J J DennisP A Sokol

Abstract

The tolQ, tolR, and tolA genes from Pseudomonas aeruginosa PAO were cloned using degenerate oligonucleotide PCR primers designed based on conserved transmembrane regions of Escherichia coli TolQ and TolR and E. coli and Pseudomonas putida ExbB and ExbD. The resulting PCR product was used as a probe to isolate a 6.5-kb DNA fragment containing P. aeruginosa tolQ, tolR, and tolA. The nucleotide sequence of a 2.9-kb DNA fragment containing the tolQ, tolR, and tolA genes was determined. The DNA sequence predicts TolQ to be a 25,250-Da protein exhibiting 53% identity to E. coli TolQ. TolR is predicted to be a 15,788-Da protein, sharing 38% identity with the E. coli TolR protein. The P. aeruginosa tolA sequence predicts a 37,813-Da protein with 27% identity to the E. coli TolA. The P. aeruginosa TolQRA proteins were expressed in E. coli minicells. Analysis of plasmid-encoded tolQ::lacZ and tolA::lacZ promoter fusions in E. coli indicated that these genes are expressed at different levels, suggesting transcription from different promoters. Transcriptional analysis of the tol genes in P. aeruginosa revealed that the tolQ and tolR genes are cotranscribed as an approximately 1.5-kb transcript and that tolA is transcribed from its own prom...Continue Reading

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Citations

Aug 6, 2014·PloS One·Alessandra Lo SciutoFrancesco Imperi
Jul 13, 2016·The Biochemical Journal·Alexander KleinColin Kleanthous
Aug 18, 2017·Molecular Microbiology·Rahul ShrivastavaShu-Sin Chng
Dec 18, 2019·Antimicrobial Agents and Chemotherapy·Shannon R ColemanRobert E W Hancock
Feb 1, 2011·Journal of Bacteriology·Christa H ChatfieldNicholas P Cianciotto
Apr 10, 2019·Antimicrobial Agents and Chemotherapy·Ryan D HeselpothVincent A Fischetti
Nov 17, 1999·Molecular Microbiology·M L Vasil, U A Ochsner
Jun 6, 2009·Microbiology and Immunology·Yang WeiKangmin Duan
Jun 14, 2014·FEMS Microbiology Reviews·Maarten G K Ghequire, René De Mot
Sep 12, 2015·The Journal of Biological Chemistry·Justyna A WojdylaColin Kleanthous
Jan 10, 2018·Molecular Microbiology·Alexander J F Egan
Feb 29, 2000·Journal of Bacteriology·A J Heilpern, M K Waldor
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
May 31, 2020·FEMS Microbiology Reviews·Joanna SzczepaniakColin Kleanthous
Mar 2, 2002·Journal of Bacteriology·Qixun Zhao, Keith Poole
Dec 18, 2002·Journal of Bacteriology·María A LlamasJosé J Rodríguez-Herva
Oct 14, 2008·BMC Genomics·Chantal W NdeWilliam E Bentley

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