Identification and characterization of the Plasmodium yoelii PyP140/RON4 protein, an orthologue of Toxoplasma gondii RON4, whose cysteine-rich domain does not protect against lethal parasite challenge infection.

Infection and Immunity
D L NarumRaul Herrera

Abstract

Previously, we identified a Plasmodium yoelii YM 140-kDa merozoite protein, designated PyP140, which formed a complex with apical membrane antigen 1 (AMA1). Furthermore, we produced a nonprotective monoclonal antibody (MAb), 48F8, that immunoprecipitated metabolically labeled PyP140 and localized the protein to the merozoite's apical end and, less frequently, to the merozoite surface, as observed by immunofluorescence assay (IFA). Here, using MAb 48F8, we have identified the pyp140 gene by screening a P. yoelii lambda-Zap cDNA expression library. The pyp140 cDNA covers approximately 90% of the putative open reading frame (ORF) of PY02159 from the P. yoelii NL genome sequencing project. Analysis of the complete gene identified the presence of two introns. The ORF encodes a 102,407-Da protein with an amino-terminal signal sequence, a series of three unique types of repeats, and a cysteine-rich region. The binding site of MAb 48F8 was also identified. A BLAST search with the deduced amino acid sequence shows significant similarity with the Toxoplasma gondii RON4 protein and the Plasmodium falciparum RON4 protein, and the sequence is highly conserved in other Plasmodium species. We produced the cysteine-rich domain of PyP140/RON4 b...Continue Reading

References

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Citations

Jun 12, 2003·Developmental and Comparative Immunology·A LalancetteM Fournier
Jul 28, 2009·The Journal of Biological Chemistry·Matthew L PlassmeyerDavid L Narum
Jan 24, 2009·PLoS Pathogens·Christine R CollinsMichael J Blackman
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Jan 18, 2013·Molecular & Cellular Proteomics : MCP·Scott E LindnerStefan H I Kappe
Feb 14, 2012·Trends in Parasitology·Daniel BargieriRobert Ménard
Jan 19, 2016·Trends in Parasitology·Greta E WeissPaul R Gilson
Mar 30, 2010·Trends in Parasitology·Nicholas I ProellocksKarena L Waller
May 16, 2009·Experimental Parasitology·Belinda J MorahanKarena L Waller
May 4, 2011·Cellular Microbiology·Sébastien BesteiroMaryse Lebrun
May 7, 2014·Cellular Microbiology·Veronica Risco-CastilloOlivier Silvie
Aug 8, 2014·Parasitology International·Joe Kimanthi MutungiOsamu Kaneko
Aug 27, 2014·International Journal for Parasitology·Katherine L HarveyBrendan S Crabb
Sep 19, 2014·PLoS Pathogens·Daniel BargieriRobert Ménard
Feb 6, 2019·Molecular & Cellular Proteomics : MCP·Anthony SiauPeter Preiser
Feb 23, 2020·Journal of Cell Science·Maria Fátima HortaThiago Castro-Gomes
May 1, 2019·Molecular & Cellular Proteomics : MCP·Anthony SiauPeter Preiser

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