PMID: 20131597Feb 6, 2010Paper

Identification and characterization of the chromium (VI) responding protein from a newly isolated Ochrobactrum anthropi CTS-325

Journal of Environmental Sciences (China)
Yangjian ChengZhang Lin

Abstract

A Gram-negative, chromium(VI) tolerant and reductive strain CTS-325, isolated from a Chinese chromate plant, was identified as Ochrobactrum anthropi based on its biochemical properties and 16S rDNA sequence analysis. It was able to tolerate up to 10 mmol/L Cr(VI) and completely reduce 1 mmol/L Cr(VI) to Cr(III) within 48 h. When the strain CTS-325 was induced with Cr(VI), a protein increased significantly in the whole cell proteins. Liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis revealed that this protein was a superoxide dismutase (SOD) homology. The measured superoxide dismutase activity was 2694 U/mg after three steps of purification. The SOD catalyzes the dismutation of the superoxide anion (O2*-) into hydrogen peroxide and molecular oxygen. This protein is considered to be one of the most important anti-oxidative enzymes for O. anthropi as it allows the bacterium to survive high oxygen stress environments, such as the environment produced during the reduction process of Cr(VI).

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Citations

Nov 6, 2013·FEMS Microbiology Reviews·Carlo VitiLuciana Giovannetti
Sep 10, 2014·Journal of Environmental Management·Hrudayanath ThatoiNigamananda Das
Jun 27, 2019·Metallomics : Integrated Biometal Science·Shruti Shah, Samir Damare
Jul 22, 2019·Journal of Hazardous Materials·Han-Wen ChenDong-Feng Liu
Mar 11, 2021·Environmental Science and Pollution Research International·Jia Chen, Yongqiang Tian
Mar 12, 2021·Journal of Environmental Management·Bhupendra PushkarNikita Nilkanth
Jul 9, 2010·Environmental Science & Technology·Yangjian ChengZiyu Wu

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