Identification and characterization of traE genes of Spiroplasma kunkelii

Gene
Xiaodong BaiSaskia A Hogenhout

Abstract

Four traE homologs, designated traE1, traE2, traE3 and traE4, were identified and amplified from the genome of the leafhopper-transmitted corn stunt pathogen Spiroplasma kunkelii and were predicted to encode membrane-bound adenine tri-phosphatases (ATPases). Deduced proteins of all traE genes have 62.3% to 89.9% similarity to the conserved VirB4 domain that is frequently a component of type IV secretory pathways involved in intracellular trafficking and secretion of DNA and proteins. In phylogenetic analysis, TraE homologs of S. kunkelii, Mycoplasma pulmonis and Mycoplasma fermentans cluster together and are more similar to TraE proteins of Gram-positive bacteria than to those of Gram-negative bacteria, thereby resembling the 16S rRNA phylogeny. Gene traE2 was most conserved whereas the presence of the three other traE genes varied among S. kunkelii strains, M2, CS-2B, FL-80 and PU8-17. Further, traE1 and traE2 appeared to be located on the chromosome, and traE3 and traE4 genes on plasmids of S. kunkelii strain M2. Transcripts of the spiralin gene and traE2 genes were detected on Northern blots containing total ribonucleic acids (RNA) of S. kunkelii cultures and S. kunkelii-infected plants and insects, in which traE2 appeared t...Continue Reading

References

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May 23, 2002·FEMS Microbiology Letters·Xiaodong Bai, Saskia A Hogenhout

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Citations

Sep 28, 2012·Phytopathology·Pablo CarpaneJacqueline Fletcher
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Cristina E Alvarez-Martinez, Peter J Christie
Nov 18, 2006·Canadian Journal of Microbiology·Ellen L DallyRobert E Davis
Jul 22, 2008·Current Opinion in Plant Biology·Saskia A Hogenhout, Rosemary Loria
Aug 19, 2008·Molecular Plant Pathology·Saskia A HogenhoutShigetou Namba
Jan 18, 2006·Molecular and Biochemical Parasitology·Sukhinder K SandhuParwinder S Grewal
Dec 21, 2016·Molecular Plant Pathology·Claire Bendix, Jennifer D Lewis
Dec 3, 2017·Applied and Environmental Microbiology·Matthew J BallingerSteve J Perlman

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