PMID: 11918670Mar 29, 2002Paper

Identification and characterization of tRNA (Gm18) methyltransferase from Thermus thermophilus HB8: domain structure and conserved amino acid sequence motifs

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
Hiroyuki HoriKimitsuna Watanabe

Abstract

Transfer RNAs from an extreme thermophile, Thermus thermophilus, commonly possess 2'-O-methylguanosine at position 18 (Gm18) in the D-loop. This modification is post-transcriptionally introduced by tRNA (Gm18) methyltransferase. Partial amino acid sequence data were obtained from purified T. thermophilus tRNA (Gm18) methyltransferase by peptide sequencing and mass spectrometry. The sequence data were used to screen the T. thermophilus genome database currently in progress, resulting in the identification of the corresponding gene. Purified recombinant enzyme showed a strict specificity for methylation at the 2'-OH of G18 in tRNA. Sequence alignment with other known or putative methyltransferases elucidates that tRNA (Gm18) methyltransferases have specific conserved region as well as three consensus motifs found in RNA ribose 2'-O-methyltransferases. The enzyme truncated at its N and C termini by limited tryptic digestion still retained binding activity for S-adenosyl-l-homocysteine, but lost the catalytic activity. This is the first report describing the identification of a methyltransferase gene of the trmH family through the analysis of a purified protein. Further, our results indicate that a restricted region(s) in the termi...Continue Reading

References

Mar 17, 1977·Nature·Y FuruichiA J Shatkin
Jan 1, 1987·Annual Review of Biochemistry·G R BjörkP M Wikström
May 1, 1987·Journal of Biochemistry·T MatsumotoK Watanabe
Jan 15, 1974·Biochemistry·P Y Chou, G D Fasman
Nov 15, 1973·European Journal of Biochemistry·J Klootwijk, R J Planta
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·I KumagaiT Oshima
Jun 25, 1994·Nucleic Acids Research·P A LimbachJ A McCloskey
Jul 1, 1993·Nucleic Acids Research·S SteinbergM Sprinzl
Mar 17, 1995·Journal of Molecular Biology·G SchluckebierX Cheng
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·E V KooninK E Rudd
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·K T TycowskiJ A Steitz
Feb 21, 1998·Nucleic Acids Research·M SprinzlS Steinberg
Feb 21, 1998·Nucleic Acids Research·J A McCloskey, P F Crain
Sep 25, 1998·The Journal of Biological Chemistry·H HoriK Watanabe
Dec 10, 1998·Nucleic Acids Research·J RozenskiJ A McCloskey
Apr 5, 2000·The Journal of Biological Chemistry·T CaldasG Richarme
Sep 13, 2000·Molecular Cell·H BüglU Jakob
Feb 1, 1959·Biochimica Et Biophysica Acta·J D SMITH, D B DUNN
Apr 1, 1997·Analytical Chemistry·A ApffelW S Hancock

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Citations

Oct 26, 2012·The Journal of Biological Chemistry·Ryota YamagamiHiroyuki Hori
Feb 9, 2012·The Journal of Experimental Medicine·Stefanie GehrigMark Helm
Jun 28, 2013·Nucleic Acids Research·Ru-Juan LiuEn-Duo Wang
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Elizabeth PlesheRobert T Batey
May 2, 2006·BMC Genomics·Stanislaw Dunin-HorkawiczJanusz M Bujnicki
Nov 24, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroshi TakedaHiroyuki Hori
Jul 25, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takashi ToyookaHiroyuki Hori
Jun 7, 2014·Frontiers in Genetics·Hiroyuki Hori
May 31, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ryota YamagamiHiroyuki Hori
Jun 16, 2016·Nucleic Acids Research·Virginie MarchandYuri Motorin
Apr 5, 2017·Biomolecules·Christian LorenzMario Mörl
May 26, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Misa NakashimaHiroyuki Hori
Sep 11, 2004·The Journal of Biological Chemistry·Hironori OkamotoHiroyuki Hori
Mar 21, 2006·The Journal of Biological Chemistry·Naoki ShigiKimitsuna Watanabe
Sep 12, 2006·The Journal of Biological Chemistry·Kazunori WatanabeHiroyuki Hori
Oct 27, 2017·Journal of Biochemistry·Chie TomikawaHiroyuki Hori
Jan 8, 2005·The Journal of Biological Chemistry·Kazunori WatanabeHiroyuki Hori
Dec 21, 2018·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Lilia AyadiYuri Motorin
May 1, 2014·EcoSal Plus·Glenn R Björk, Tord G Hagervall
Nov 1, 2005·EcoSal Plus·Glenn R Björk, Tord G Hagervall
Jun 15, 2021·Advanced Biology·Maik SchauerteKatharina Höfer

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