Identification and characterization of two distinct GnRH receptor subtypes in a teleost, the medaka Oryzias latipes

Endocrinology
Kataaki OkuboKatsumi Aida

Abstract

We report the identification and characterization of two distinct GnRH receptor (GnRH-R) subtypes, designated GnRH-R1 and GnRH-R2, in a model teleost, the medaka Oryzias latipes. These seven-transmembrane receptors of the medaka contain a cytoplasmic C-terminal tail, which has been found in all other nonmammalian GnRH-Rs cloned to date. The GnRH-R1 gene is composed of three exons separated by two introns, whereas the GnRH-R2 gene has an additional intron and therefore consists of four exons and three introns. The GnRH-R1 and GnRH-R2 genes, both of which exist as single-copy genes in the medaka genome, were mapped to linkage groups 3 and 16, respectively. Inositol phosphate assays using COS-7 cells transfected with GnRH-R1 and GnRH-R2 demonstrated that they had remarkably different ligand sensitivities, although both receptors showed highest preference for chicken-II-type GnRH. Phylogenetic analysis showed the presence of three paralogous lineages for vertebrate GnRH-Rs and indicated that neither GnRH-R1 nor GnRH-R2 is the medaka ortholog to mammalian GnRH-Rs that lack a cytoplasmic tail. This, together with an observation that medaka-type GnRH had low affinity for GnRH-R1 and GnRH-R2, suggests that a third GnRH-R may exist in t...Continue Reading

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Citations

Aug 1, 2012·PloS One·Takehiro G KusakabeMotoyuki Tsuda
Feb 6, 2017·General and Comparative Endocrinology·Sanny David Pacheco LumaynoMichiya Matsuyama
Jul 26, 2002·Gene·Kataaki OkuboKatsumi Aida
Apr 1, 2005·Fish Physiology and Biochemistry·Ishwar S Parhar
Jun 7, 2002·Endocrine Reviews·Chandra P LeoAaron J W Hsueh
Apr 15, 2004·Endocrine Reviews·Robert P MillarStuart R Maudsley
Sep 12, 2015·Journal of Neurophysiology·Chie UmataniYoshitaka Oka
Feb 11, 2020·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·Joseph R D FernandesArnab Banerjee
Jun 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Md Rajib SharkerKang Hee Kho
Feb 8, 2020·General and Comparative Endocrinology·José A Muñoz-CuetoYonathan Zohar
Jan 5, 2011·General and Comparative Endocrinology·Rosanna ChianeseGilda Cobellis
Jun 11, 2002·Biochemical and Biophysical Research Communications·Kataaki OkuboKatsumi Aida
Mar 8, 2008·Environmental Science & Technology·Abimael LeónSwee J Teh

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