Identification and characterization of virus assembly intermediate complexes in HIV-1-infected CD4+ T cells

Virology
Y M Lee, X F Yu

Abstract

For type-C and lentiviruses, including human immunodeficiency virus type 1 (HIV-1), the pathway of virus assembly remains poorly defined, and the assembly and budding of capsids are believed to occur simultaneously at the plasma membrane of the infected cell. We have now identified two putative HIV-1 assembly intermediate complexes in infected CD4+ T cells. The first of these intermediates, a detergent-resistant complex (DRC), was identified as a large oligomer that had a density of 1.10-1.13 g/ml and was primarily composed of Pr55Gag and Pr160Gag-Pol precursors. The other putative intermediate was a detergent-sensitive complex (DSC) with a density of 1.15-1.17 g/ml, which apparently represented the products of extensive proteolytic processing of both the Pr55Gag and Pr160Gag-Pol precursors. Both complexes could be distinguished from released mature virions as well as immature viral particles. Surprisingly, the formation of DRC was not dependent upon the myristylation at the N-terminus of the Gag proteins, a signal required for plasma membrane targeting and virus production. However, the myristic acid modification was essential for the formation of DSC. These data suggest that interactions between individual Gag molecules and b...Continue Reading

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Citations

Nov 16, 2001·AIDS Research and Human Retroviruses·F J IbarrondoP Krogstad
Oct 26, 2010·AIDS Research and Human Retroviruses·María L RauddiSilvia A González
Jul 5, 2007·Journal of Virology·Stefanie JägerHans-Georg Kräusslich
Nov 9, 2000·Journal of Virology·A Ohagen, D Gabuzda
Aug 6, 2005·Retrovirology·Gilles AudolyPablo Gluschankof
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·A Ono, E O Freed
Aug 19, 2015·The Journal of Cell Biology·Jelle HendrixDon C Lamb
Mar 30, 2010·Virus Research·José L Affranchino, Silvia A González
May 24, 2014·The Journal of General Virology·Juan C Abdusetir CerfoglioJosé L Affranchino
Mar 17, 2009·Virology·Jun DouPaul Spearman
Jul 30, 2014·Virus Research·Jaisri R LingappaBridget A Robinson
May 1, 2006·Current Opinion in HIV and AIDS·Paul Spearman
May 21, 2004·The Journal of Biological Chemistry·Yuko MorikawaFumitaka Momose
Mar 18, 2004·Reviews in Medical Virology·Catherine S Adamson, Ian M Jones
Feb 5, 2021·Journal of Molecular Biology·Yisong DengJames R Williamson

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