Identification and characterization of ceftriaxone resistance and extended-spectrum beta-lactamases in Malawian bacteraemic Enterobacteriaceae

The Journal of Antimicrobial Chemotherapy
Katherine J GrayC Anthony Hart

Abstract

To enumerate and characterize extended-spectrum beta-lactamases (ESBLs) amongst ceftriaxone-resistant coliforms in Blantyre, Malawi, where third-generation cephalosporin use is currently highly restricted. Over the period April 2004-March 2005 all ceftriaxone-resistant isolates from blood cultures were examined for the presence of ESBLs. Isoelectric focusing was performed on enzyme extracts. PCR and DNA sequencing of amplicons were used to identify the underlying genetic determinants responsible for the ESBL phenotypes. Transferability of the ESBL phenotypes was tested by conjugation to a susceptible Escherichia coli J53. Enterobacteriaceae were isolated from 1191 blood cultures, of which 19 (1.6%) were ceftriaxone resistant. Ten isolates (0.7% of all isolates) demonstrated an ESBL phenotype but only eight were characterized as three isolates were from the same patient. Genotypes SHV-11 (n = 1), SHV-12 (n = 3), SHV-27 (n = 1), TEM-63 (n = 2) and CTX-M-15 (n = 1) were detected. Plasmid transfer of the ESBL resistance phenotype was successful for all the isolates. In a clinical setting of minimal cephalosporin usage there is already a diversity of ESBL genotypes. Increased use of cephalosporins in this setting is likely to result...Continue Reading

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Citations

Jan 14, 2010·Annals of Clinical Microbiology and Antimicrobials·Murat KasapHaluk Vahaboglu
Jan 15, 2010·Annals of Clinical Microbiology and Antimicrobials·Shazi Shakil, Asad U Khan
Oct 24, 2014·Emerging Infectious Diseases·Nicholas A FeaseyRobert A Kingsley
Mar 24, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Liam BurkeDeirdre Fitzgerald-Hughes
May 26, 2016·International Journal of Medical Microbiology : IJMM·Daniel EibachJürgen May
Mar 24, 2017·The Journal of Antimicrobial Chemotherapy·Patrick MusichaChisomo L Msefula
Sep 12, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Kittitat LugsomyaNuvee Prapasarakul
Nov 21, 2009·Journal of Clinical Laboratory Analysis·Zhijian ZhangQinxian Zhang
Jul 2, 2008·Biological & Pharmaceutical Bulletin·Marjorie Moreira GuedesVietla Satyanarayana Rao
Jan 10, 2012·Asian Pacific Journal of Tropical Medicine·Shahzad F HaqueAsad U Khan
Jun 15, 2021·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Onduru G OnduruSusan F Rumisha

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