Identification and characterization of Rhipicephalus (Boophilus) microplus candidate protective antigens for the control of cattle tick infestations.

Parasitology Research
Consuelo AlmazánJosé de la Fuente

Abstract

The cattle ticks, Rhipicephalus (Boophilus) spp., affect cattle production in tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The recombinant Rhipicephalus microplus Bm86 antigen has been shown to protect cattle against tick infestations. However, variable efficacy of Bm86-based vaccines against geographic tick strains has encouraged the research for additional tick-protective antigens. Herein, we describe the analysis of R. microplus glutathione-S transferase, ubiquitin (UBQ), selenoprotein W, elongation factor-1 alpha, and subolesin (SUB) complementary DNAs (cDNAs) by RNA interference (RNAi) in R. microplus and Rhipicephalus annulatus. Candidate protective antigens were selected for vaccination experiments based on the effect of gene knockdown on tick mortality, feeding, and fertility. Two cDNA clones encoding for UBQ and SUB were used for cattle vaccination and infestation with R. microplus and R. annulatus. Control groups were immunized with recombinant Bm86 or adjuvant/saline. The highest vaccine efficacy for the control of tick infestations was obtained for Bm86. Although with low immunogenic response, the results with the S...Continue Reading

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Citations

Feb 12, 2011·BMC Genomics·Lorena TorresJosé de la Fuente
Feb 10, 2011·Journal of Visualized Experiments : JoVE·Katherine M KocanJosé de la Fuente
Feb 20, 2014·Ticks and Tick-borne Diseases·Georg Gerhard DuscherJosé de la Fuente
Jul 7, 2012·The Veterinary Journal·Luís F PariziItabajara da Silva Vaz
May 3, 2012·International Journal for Parasitology·Felix D GuerreroAdalberto A Pérez de León
Jan 10, 2016·Ticks and Tick-borne Diseases·Tae K KimAlbert Mulenga
Dec 7, 2014·Veterinary Parasitology·Abid AliCarlos Termignoni
Jun 23, 2015·Ticks and Tick-borne Diseases·Sabine A RichardsChristine Maritz-Olivier
Feb 26, 2013·Cellular Microbiology·Maiara S SeveroJoao H F Pedra
Mar 5, 2014·Transboundary and Emerging Diseases·J de la FuenteE F Blouin
Jul 30, 2014·Parasite Immunology·E J MarrS T G Burgess
Jan 8, 2014·Vaccine·José de la Fuente, Octavio Merino
Jan 30, 2014·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Rodrigo Casquero CunhaAlexandre Leitão
Apr 6, 2013·Revista Da Sociedade Brasileira De Medicina Tropical·Ana DomingosVirgílio Estólio do Rosário
Mar 31, 2016·PLoS Neglected Tropical Diseases·Vladimir LópezJosé de la Fuente
Mar 30, 2018·Frontiers in Cellular and Infection Microbiology·Christian StutzerChristine Maritz-Olivier

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Datasets Mentioned

BETA
AF077609
AA257892
EU436163
DQ159966
GQ456170

Methods Mentioned

BETA
gene knockdown
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

Excel

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